Diagnostic significance of urinary long non-coding PCA3 RNA in prostate cancer.
Diagnostic significance of urinary long non-coding PCA3 RNA in prostate cancer.
复制标题
尿长链非编码 PCA3 RNA 对前列腺癌的诊断意义。
DOI：
10.18632/oncotarget.17272
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发表时间：
2017-08-29
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Wang T;Qu X;Jiang J;Gao P;Zhao D;Lian X;Li X
前列腺癌抗原3(PCA3)是一种非编码的长链RNA，在CAP患者中的表达明显高于正常人。本研究以PCA3和PSA单独核酸的重组表达为参比试剂，建立了检测尿液中PCA3和PSA基因拷贝数的TaqMan定量RT-PCR方法。结果表明，PCA3拷贝数、PSA拷贝数和PCA3/PSARNA评分的受试者工作特征曲线下面积分别为0.717、0.444和0.916。此外，血清tPSA的AUROC值为0.674，特异度为12.07%。最后，通过一项多中心临床试验，对PCA3RNA与PSA RNA的算法进行了评估，并证实其为CAP生物标记物。本研究不仅验证了建立的检测尿PCA3和PSA RNA的定量逆转录聚合酶链式反应(QRT-PCR)和TaqMan法，还证实了Pca/PSA RNA评分是诊断CAP的可靠指标。
Prostate cancer antigen 3(PCA3) is a long non-coding RNA, which was found increased expression in CaP patients than healthy individual. In this study, the individual nucleic acid of PCA3 and PSA was recombinant expressed as a reference reagent, and a quantitative RT-PCR with TaqMan assay was developed to examine the copies of PCA3 and PSA gene in urine. The results showed that the area under the receiver operating characteristic curve (AUROC) was 0.717, 0.444 and 0.916 for the number of PCA3 copy, PSA copy and for the score of PCA3/PSA RNA, respectively. Additionally, the AUROC for serum tPSA was 0.674 with a low specificity of 12.07%. Finally, the algorithm of PCA3 RNA versus PSA RNA was evaluated and corroborated as CaP biomarker by conducting a multicentric clinical trial. This study not only validated the developed technique of qRT-PCR with TaqMan assay for examination of urinary PCA3 and PSA RNA, it also demonstrated that the score of the PCA/PSA RNA was a reliable signature for CaP diagnosis.
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影响因子：
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