ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections.
ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections.
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DOI：
10.1016/j.xpro.2021.100696
复制
发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Atkinson MA
中科院分区：
文献类型：
--
作者：
Jorgensen M;Joseph P;Posgai AL;Vander Heide RS;Kusmartseva I;Atkinson MA
血管紧张素转换酶2（ACE 2）是严重急性呼吸综合征冠状病毒2的关键细胞进入因子。因此，鉴定表达ACE2的细胞类型对于了解2019冠状病毒病的病理生理学非常重要。我们对各种人体组织类型的多种一抗进行了广泛的测试。在这里，我们描述了一个优化的协议，从器官捐献者和尸检获得的福尔马林固定石蜡包埋的人胰腺，小肠和肾脏组织切片的ACE2免疫染色。有关本方案使用和执行的完整详细信息，请参阅。我们提出了一种兔单克隆IgG抗ACE 2（克隆EPR 4435）的免疫染色方案（2）ACE 2染色已在人胰腺、小肠和肾脏中进行了优化。方案已使用来自人体器官供体和尸检的组织切片进行了优化。血管紧张素转换酶2（ACE 2）是严重急性呼吸综合征冠状病毒2的关键细胞进入因子。因此，鉴定表达ACE2的细胞类型对于了解2019冠状病毒病的病理生理学非常重要。我们对各种人体组织类型的多种一抗进行了广泛的测试。在这里，我们描述了一个优化的协议，从器官捐献者和尸检获得的福尔马林固定石蜡包埋的人胰腺，小肠和肾脏组织切片的ACE2免疫染色。
Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies. For complete details on the use and execution of this protocol, please refer to. We present an immunostaining protocol for rabbit monoclonal IgG anti-ACE2, clone EPR4435(2) ACE2 staining has been optimized in human pancreas, small intestine, and kidney Protocol has been optimized using tissue sections from human organ donors and autopsies Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies.
影响因子：
7.3
作者：
Hamming, I;Timens, W;van Goor, H
通讯作者：
van Goor, H
影响因子：
29
作者：
Kusmartseva I;Wu W;Syed F;Van Der Heide V;Jorgensen M;Joseph P;Tang X;Candelario-Jalil E;Yang C;Nick H;Harbert JL;Posgai AL;Paulsen JD;Lloyd R;Cechin S;Pugliese A;Campbell-Thompson M;Vander Heide RS;Evans-Molina C;Homann D;Atkinson MA
通讯作者：
Atkinson MA
DOI：
10.1007/978-1-4939-8935-5_22
发表时间：
2019-01-01
期刊：
BIOBANKING
影响因子：
--
作者：
Sadeghipour, Alireza;Babaheidarian, Pegah
通讯作者：
Babaheidarian, Pegah