Circadian rhythms of cyanobacteria: monitoring the biological clocks of individual colonies by bioluminescence
Circadian rhythms of cyanobacteria: monitoring the biological clocks of individual colonies by bioluminescence
复制标题
蓝藻的昼夜节律：通过生物发光监测单个菌落的生物钟
作者：
Takao Kondo;M. Ishiura
蓝藻(聚球藻)个体菌落生物发光的可重复性昼夜节律菌株PCC7942)。琼脂平板上菌落的表型监测将使我们能够通过筛选时钟突变来从遗传学上分析蓝藻生物钟的分子机制。通过引入细菌荧光素酶基因，我们先前培育了一株转化的蓝藻菌株(AMC149)，该菌株表达荧光素酶作为生物钟的生物发光报告。在液体培养中，AMC149表达的生物发光节律表现出与高等真核生物的昼夜节律相同的行为。在给予报告酶底物(癸醛)的技术上的改进和高灵敏度的光子计数照相机允许监测单个菌落的生物发光。琼脂平板上的单个菌落表现出与先前报道的液体培养基本相同的节律性。
Reproducible circadian rhythms of bioluminescence from individual colonies of cyanobacteria (Synechococcus sp. strain PCC 7942) has been observed. Phenotypic monitoring of colonies on agar plates will enable us to genetically analyze the molecular mechanism of the circadian clock of cyanobacteria by screening for clock mutants. By the introduction of a bacterial luciferase gene, we previously developed a transformed cyanobacterial strain (AMC149) that expresses luciferase as a bioluminescent reporter of the circadian clock. In liquid culture, AMC149 expresses a rhythm of bioluminescence that displays the same behavior as circadian rhythms in higher eukaryotes. Improvements in the technique for administering the reporter enzyme's substrate (decanal) and a highly sensitive photon-counting camera allow monitoring the bioluminescence of single colonies. Individual colonies on agar plates displayed a rhythmicity which is essentially the same as that previously reported for liquid cultures.
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影响因子：
56.9
作者：
MICHEL, S;GEUSZ, ME;BLOCK, GD
通讯作者：
BLOCK, GD
影响因子：
2.9
作者：
BALDWIN, TO;BERENDS, T;ZIEGLER, MM
通讯作者：
ZIEGLER, MM
DOI：
10.1073/pnas.90.12.5672
发表时间：
1993-06-15
影响因子：
11.1
作者：
KONDO, T;STRAYER, CA;JOHNSON, CH
通讯作者：
JOHNSON, CH
振荡分子以及它们如何使生物钟跨越进化界限。
DOI：
10.1073/pnas.90.12.5382
发表时间：
1993
影响因子：
11.1
作者：
Hall,JC;Rosbash,M
通讯作者：
Rosbash,M