Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.
Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.
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不同刺激后小鼠腹膜巨噬细胞的精确 N-糖蛋白组学分析
DOI：
10.3389/fimmu.2021.722293
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发表时间：
2021
影响因子：
7.3
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Yang L;Gong T;Shen H;Pei J;Zhang L;Zhang Q;Huang Y;Hu Z;Pan Z;Yang P;Lin L;Yu H
巨噬细胞是重要的免疫细胞，参与先天性和适应性免疫反应，例如吞噬作用、分子模式识别和免疫反应激活。在这项研究中，分离了小鼠腹腔巨噬细胞，然后用 LPS、HSV 和 VSV 激活。进行综合蛋白质组学和精确 N-糖蛋白质组学分析来评估潜在的巨噬细胞激活。我们在糖蛋白质组学中总共鉴定了 587 种糖蛋白，其中包括 1239 种糖肽、526 种单糖成分和 8326 种完整糖肽，以及在蛋白质组学分析中总共鉴定了 4496 种蛋白质。这些糖蛋白广泛参与重要的生物过程，例如抗原呈递、细胞因子产生和糖基化进程。在不同病原体的刺激下，糖蛋白表现出巨大的变化。我们发现Toll样受体途径中的受体，例如Tlr2和CD14，在LPS和HSV刺激下增加。这些蛋白质的糖基化被证明会影响它们的亚细胞位置。
Macrophages are important immune cells that participate in both innate and adaptive immune responses, such as phagocytosis, recognition of molecular patterns, and activation of the immune response. In this study, murine peritoneal macrophages were isolated and then activated by LPS, HSV and VSV. Integrative proteomic and precision N-glycoproteomic profiling were conducted to assess the underlying macrophage activation. We identified a total of 587 glycoproteins, including 1239 glycopeptides, 526 monosaccharide components, and 8326 intact glycopeptides in glycoproteomics, as well as a total of 4496 proteins identified in proteomic analysis. These glycoproteins are widely involved in important biological processes, such as antigen presentation, cytokine production and glycosylation progression. Under the stimulation of the different pathogens, glycoproteins showed a dramatic change. We found that receptors in the Toll-like receptor pathway, such as Tlr2 and CD14, were increased under LPS and HSV stimulation. Glycosylation of those proteins was proven to influence their subcellular locations.
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影响因子：
5.2
作者：
Brun P;Scarpa M;Marchiori C;Conti J;Kotsafti A;Porzionato A;De Caro R;Scarpa M;Calistri A;Castagliuolo I
通讯作者：
Castagliuolo I
影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
4.4
作者：
Gangloff, SC;Zähringer, U;Goyert, SM
通讯作者：
Goyert, SM
影响因子：
8.6
作者：
Lu, Si-Min;Wu, Xue-Yuan;Long, Yi-Tao
通讯作者：
Long, Yi-Tao
影响因子：
64.5
作者：
Lavin Y;Winter D;Blecher-Gonen R;David E;Keren-Shaul H;Merad M;Jung S;Amit I
通讯作者：
Amit I