Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm.
Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm.
复制标题
DOI：
10.3389/fpls.2017.01555
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
Uauy C
中科院分区：
文献类型：
--
作者：
Shorinola O;Balcárková B;Hyles J;Tibbits JFG;Hayden MJ;Holušova K;Valárik M;Distelfeld A;Torada A;Barrero JM;Uauy C
Pre-harvest sprouting (PHS) is an important cause of quality loss in many cereal crops and is particularly prevalent and damaging in wheat. Resistance to PHS is therefore a valuable target trait in many breeding programs. The Phs-A1 locus on wheat chromosome arm 4AL has been consistently shown to account for a significant proportion of natural variation to PHS in diverse mapping populations. However, the deployment of sprouting resistance is confounded by the fact that different candidate genes, including the tandem duplicated Plasma Membrane 19 (PM19) genes and the mitogen-activated protein kinase kinase 3 (TaMKK3-A) gene, have been proposed to underlie Phs-A1. To further define the Phs-A1 locus, we constructed a physical map across this interval in hexaploid and tetraploid wheat. We established close proximity of the proposed candidate genes which are located within a 1.2 Mb interval. Genetic characterization of diverse germplasm used in previous genetic mapping studies suggests that TaMKK3-A, and not PM19, is the major gene underlying the Phs-A1 effect in European, North American, Australian and Asian germplasm. We identified the non-dormant TaMKK3-A allele at low frequencies within the A-genome diploid progenitor Triticum urartu genepool, and show an increase in the allele frequency in modern varieties. In United Kingdom varieties, the frequency of the dormant TaMKK3-A allele was significantly higher in bread-making quality varieties compared to feed and biscuit-making cultivars. Analysis of exome capture data from 58 diverse hexaploid wheat accessions identified fourteen haplotypes across the extended Phs-A1 locus and four haplotypes for TaMKK3-A. Analysis of these haplotypes in a collection of United Kingdom and Australian cultivars revealed distinct major dormant and non-dormant Phs-A1 haplotypes in each country, which were either rare or absent in the opposing germplasm set. The diagnostic markers and haplotype information reported in the study will help inform the choice of germplasm and breeding strategies for the deployment of Phs-A1 resistance into breeding germplasm.
登录
查看更多内容
影响因子：
12.3
作者：
Krasileva KV;Buffalo V;Bailey P;Pearce S;Ayling S;Tabbita F;Soria M;Wang S;IWGS Consortium;Akhunov E;Uauy C;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
2.1
作者：
Flintham, JE
通讯作者：
Flintham, JE
影响因子：
1.9
作者：
Knox, R. E.;Clarke, F. R.;Singh, A. K.
通讯作者：
Singh, A. K.
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf
影响因子：
12.3
作者：
Barrero JM;Cavanagh C;Verbyla KL;Tibbits JF;Verbyla AP;Huang BE;Rosewarne GM;Stephen S;Wang P;Whan A;Rigault P;Hayden MJ;Gubler F
通讯作者：
Gubler F