Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro.
Reduced growth and proliferation dynamics of nasal epithelial stem/progenitor cells in nasal polyps in vitro.
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鼻息肉中鼻上皮干/祖细胞的体外生长和增殖动力学降低
DOI：
10.1038/srep04619
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发表时间：
2014-04-09
影响因子：
4.6
通讯作者：
Wang de Y
中科院分区：
文献类型：
--
作者：
Yu XM;Li CW;Chao SS;Li YY;Yan Y;Zhao XN;Yu FG;Liu J;Shen L;Pan XL;Shi L;Wang de Y
Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67+ cells among p63+ cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.
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影响因子：
14.2
作者：
Li, Chun Wei;Shi, Li;Wang, De Yun
通讯作者：
Wang, De Yun
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
12.4
作者：
Mansson, A.;Bogefors, J.;Cardell, L. O.
通讯作者：
Cardell, L. O.
影响因子：
14.2
作者：
Tieu, David D.;Peters, Anju T.;Carter, Roderick T.;Suh, Lydia;Conley, David B.;Chandra, Rakesh;Norton, James;Grammer, Leslie C.;Harris, Kathleen E.;Kato, Atsushi;Kern, Robert C.;Schleimer, Robert P.
通讯作者：
Schleimer, Robert P.
影响因子：
10
作者：
Fedorov, IA;Wilson, SJ;Holgate, ST
通讯作者：
Holgate, ST