Degradation, cytotoxicity, and biocompatibility of NIPAAm-based thermosensitive, injectable, and bioresorbable polymer hydrogels.
Degradation, cytotoxicity, and biocompatibility of NIPAAm-based thermosensitive, injectable, and bioresorbable polymer hydrogels.
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DOI：
10.1002/jbm.a.33093
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发表时间：
2011-08
影响因子：
4.9
通讯作者：
Vernon, Brent L.
中科院分区：
文献类型：
--
作者：
Cui, Zhanwu;Lee, Bae Hoon;Pauken, Christine;Vernon, Brent L.
采用自由基共聚法合成了一种温敏性、可注射性和生物可吸收性的聚合物水凝胶--聚（N-异丙基丙烯酰胺-co-二甲基-γ-丁内酯丙烯酸酯-co-丙烯酸）（poly（NDBA）），其质量分数为7.00 mol.%二甲基-γ-丁内酯丙烯酸酯在四氢呋喃（THF）中的溶液。通过酸滴定结合1H NMR定量测定化学组成。通过凝胶渗透色谱法（GPC）结合静态光散射测定分子量和多分散性。采用差示扫描量热法（DSC）、质量损失率、浊点试验和溶胀率随时间的变化对聚合物水凝胶的降解性能进行了表征。发现聚合物的初始LCST在室温和体温之间，并且在生理条件下LCST超过体温需要约2周。间接细胞毒性试验表明，该共聚物具有相对较低的细胞毒性，如3 T3成纤维细胞所示。体内凝胶化和降解研究显示与体外降解结果具有良好的一致性，并且在聚合物完全溶解后未观察到对邻近组织的有害影响。
A thermosensitive, injectable and bioresorbable polymer hydrogel, Poly(N-isopropylacrylamide-co-dimethyl-γ-butyrolactone acrylate-co-acrylic acid) (poly(NDBA)), was synthesized by radical copolymerization with 7.00 mol.% dimethyl-γ-butyrolactone acrylate in tetrahydrofuran (THF). The chemical composition was determined by acid titration in conjunction with 1H NMR quantification. The molecular weight and polydispersity were determined by gel permeation chromatography (GPC) in conjunction with static light scattering. The degradation properties of the polymer hydrogel were characterized using differential scanning calorimetry (DSC), percentage mass loss, cloud point test and swelling ratio over time. It was found that the initial LCST of the polymer is between room temperature and body temperature and that it takes about 2 weeks for the LCST to surpasses body temperature under physiological conditions. An indirect cytotoxicity test indicated that this copolymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells. The in vivo-gelation and degradation study showed good agreement with in vitro-degradation findings and no detrimental effects to adjacent tissues were observed after the complete dissolution of the polymer.
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