Efficient modulation of photosynthetic apparatus confers desiccation tolerance in the resurrection plant Boea hygrometrica
Efficient modulation of photosynthetic apparatus confers desiccation tolerance in the resurrection plant Boea hygrometrica
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光合机构的有效调节赋予复活植物Boea hygrometrica的干燥耐受性
DOI：
10.1093/pcp/pcx140
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发表时间：
2017-11
影响因子：
4.9
通讯作者：
Lin Honghui
中科院分区：
文献类型：
--
作者：
Tan Tinghong;Sun Yanni;Luo Shishuai;Zhang Chao;Zhou Huapeng;Lin Honghui
Boea hygrometrica（B.湿度计）可以耐受严重的干燥，并在水可用时迅速恢复光合活性。然而，关于B. hygrometrica适应脱水和恢复能力后再水化。这里我们确定B. hygrometrica涉及脱水/再水化过程中的氧化应激、过度激发/电子压力以及光合机构调节。通过测定活性氧的产生和清除率，发现B. hygrometrica具有维持细胞氧化还原稳态的有效策略。透射电子显微镜（TEM）分析显示，在脱水/复水过程中叶绿体结构和质体微球（PGs）积累显着的变化。对B叶片进行了脉冲幅度调制（PAM）叶绿素荧光测定、P700氧化还原分析和叶绿素荧光发射光谱分析。还进行了脱水/再水化期间的湿度测定。结果表明，在B中，PSII的光化学活性和光保护能量耗散在脱水/复水过程中经历了逐渐失活/活化的过程。PSI活性在水分亏缺时相对地被诱导，并且脱水导致PSI和LHCII之间的物理相互作用。此外，蓝色聚丙烯酰胺凝胶电泳（BN-PAGE）和免疫印迹分析表明，随着叶片内部水分亏缺，捕光复合物的蛋白质丰度显著沿着降低，限制了叶片对光的吸收，减弱了电子传递，从而限制了叶片的光激发，抑制了叶片的光合作用。相反，许多类囊体蛋白即使在完全脱水后仍保持在基础水平。综上所述，我们的研究表明，细胞氧化还原稳态和光合活性的有效调节赋予了B的脱水耐受性。测湿仪
Boea hygrometrica (B. hygrometrica) can tolerate severe desiccation and resume photosynthetic activity rapidly upon water availability. However, little is known about the mechanisms by which B. hygrometrica adapts to dehydration and resumes competence upon rehydration. Here we determine how B. hygrometrica deals with oxidative stress, excessive excitation/electron pressures as well as photosynthetic apparatus modulation during dehydration/rehydration. By measuring ROS generation and scavenging efficiency, we found that B. hygrometrica possesses efficient strategies to maintain cellular redox homeostasis. Transmission electron microscopy (TEM) analysis revealed a remarkable alteration of chloroplast architecture and plastoglobules (PGs) accumulation during dehydration/rehydration. Pulse-amplitude modulated (PAM) chlorophyll fluorescence measurements, P700 redox assay as well as chlorophyll fluorescence emission spectra analysis on leaves of B. hygrometrica during dehydration/rehydration were also performed. Results showed that the photochemical activity of PSII as well as photoprotective energy dissipation in PSII undergo gradual inactivation/activation during dehydration/rehydration in B. hygrometrica; PSI activity is relatively induced upon water deficit, and dehydration leads to physical interaction between PSI and LHCII. Furthermore, blue-native polyacrylamide gel electrophoresis (BN-PAGE) and immunoblot analysis revealed that the protein abundance of light harvesting complexes decrease markedly along with internal water deficit to restrict light absorption and attenuate electron transfer, resulting in limited light excitation and repressed photosynthesis. In contrast, many thylakoid proteins remain at a basal level even after full dehydration. Taken together, our study demonstrated that efficient modulation of cellular redox homeostasis and photosynthetic activity confers desiccation tolerance in B. hygrometrica.
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