Reliable and disposable quantum dot–based electrochemical immunosensor for aflatoxin B1 simplified analysis with automated magneto-controlled pretreatment system
Reliable and disposable quantum dot–based electrochemical immunosensor for aflatoxin B1 simplified analysis with automated magneto-controlled pretreatment system
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可靠且一次性的基于量子点的电化学免疫传感器，用于黄曲霉毒素 B1，通过自动磁控预处理系统简化分析
DOI：
10.1007/s00216-020-02897-x
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发表时间：
2020-08
影响因子：
4.3
通讯作者：
Songxue Wang
中科院分区：
文献类型：
--
作者：
Zhihong Xuan;Hongmei Liu;Jin Ye;Li Li;Wei Tian;Songxue Wang
成功研制了基于量子点电化学免疫传感器和磁控自动化预处理系统的黄曲霉毒素B1（AFB1）集成检测平台。自动化预处理系统采用免疫亲和磁珠（IMB）作为afb1的捕获探针，qd标记的afb1完全抗原（AFB1-BSA-QDs）作为信号探针。AFB1-BSA-QDs通过酸性处理可以很容易地转化为相应的金属阳离子，可以通过阳极溶出伏安法（ASV）进行电化学检测。此外，该新型电化学免疫传感器采用一次性丝网印刷电极（SPE），无需进一步修饰，使常规检测成为可能。在最佳条件下，afb1的检测浓度范围为0.08 ~ 800 μg/kg。在5 ~ 240 μg/kg范围内，金属离子信号与afb1浓度的对数呈线性相关，检出限为0.05 μg/kg。3种不同浓度的加标回收率在83.9 ~ 118.0%之间，日内相对标准偏差均小于10%。此外，通过对自然污染样品的分析，验证了该方法的有效性，结果与LC-MS/MS的结果吻合良好，证明了该方法用于监测谷物和油脂中afb1的潜力。
An integrated aflatoxin B1(AFB1) detection platform with quantum dot (QD)-based electrochemical immunosensor and an automated magneto-controlled pretreatment system was successfully developed. The automated pretreatment system adopts the immunoaffinity magnetic beads (IMB) as the capture probe of AFB1and QD-labeled AFB1complete antigen (AFB1-BSA-QDs) as the signal probe. AFB1-BSA-QDs can be easily converted into corresponding metallic cations through acidic treatment, which can be detected electrochemically via anode stripping voltammetry (ASV). Moreover, a disposable screen-printed electrode (SPE) without requiring any further modification is used in the novel electrochemical immunosensor' making routine testing feasible. Under optimal conditions, the detectable concentration range of AFB1was 0.08-800 μg/kg. The metal ion signal associated linearly with the logarithm of AFB1concentration within the range of 5-240 μg/kg, with a detection limit of 0.05 μg/kg. The spiked recoveries of three different concentrations in four different matrixes ranged from 83.9 to 118.0%, and inter-day relative standard deviations were below 10%. Furthermore, the methodology was validated by analyzing naturally contaminated samples, and results of the novel immunosensor were in good agreement with those of LC-MS/MS, demonstrating the potentiality of the developed method for the monitor of AFB1in cereals and oils.
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