Messenger RNA expression patterns in bovine embryos derived from in vitro procedures and their implications for development.
Messenger RNA expression patterns in bovine embryos derived from in vitro procedures and their implications for development.
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来自体外程序的牛胚胎中的信使 RNA 表达模式及其对发育的影响。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
H. Niemann
中科院分区：
文献类型：
--
作者：
C. Wrenzycki;D. Herrmann;A. Lucas;K. Korsawe;E. Lemme;H. Niemann
植入前牛胚胎最初是在卵子发生过程中积累的母体基因组信息的控制下。发育的遗传程序很快就依赖于胚胎基因组激活产生的新转录物。发育的早期步骤，包括第一次卵裂的时间，胚胎基因组的激活，致密化和囊胚形成，可能会受到培养基和条件以及生产过程本身的影响。这些扰动可能导致囊胚质量的显著下降，甚至可能影响移植后出生的后代的生存能力。体外程序，如牛胚胎的体外生产和体细胞核移植，已被证明与显著上调或下调、从头诱导或沉默对不受干扰的胎儿和新生儿发育至关重要的基因相关。这些改变可能是由表观遗传修饰引起的，如DNA甲基化和组蛋白修饰。分析扰动的表观遗传重编程和相关的现象，如基因组印记和X染色体失活，在牛胚胎是有希望的了解发育异常的潜在机制，如大后代综合征。
The preimplantation bovine embryo is initially under the control of maternal genomic information that is accumulated during oogenesis. The genetic programme of development soon becomes dependent on new transcripts derived from activation of the embryonic genome. The early steps in development, including the timing of the first cleavage, activation of the embryonic genome, compaction and blastocyst formation, can be affected by the culture media and conditions, as well as the production procedure itself. These perturbations can possibly result in a marked decrease in the quality of the resulting blastocysts and may even affect the viability of offspring born after transfer. In vitro procedures such as in vitro production and somatic nuclear transfer of bovine embryos have been shown to be correlated with significant up- or downregulation, de novo induction or silencing of genes critical for undisturbed fetal and neonatal development. These alterations are likely to be caused by epigenetic modifications, such as DNA methylation and histone modifications. Analysis of perturbed epigenetic reprogramming and of the related phenomena, such as genomic imprinting and X-chromosome inactivation, in bovine embryos is promising for understanding the underlying mechanisms of developmental abnormalities, such as large offspring syndrome.
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通过差异显示 RT-PCR 评估牛囊胚基因表达模式对培养环境的敏感性。
DOI：
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发表时间：
2001
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Natale,DR;DeSousa,PA;Westhusin,ME;Watson,AJ
通讯作者：
Watson,AJ
影响因子：
9.8
作者：
DeBaun, MR;Niemitz, EL;Feinberg, AP
通讯作者：
Feinberg, AP
影响因子：
3.5
作者：
Killian, JK;Nolan, CM;Jirtle, RL
通讯作者：
Jirtle, RL
DOI：
10.1073/pnas.0306846101
发表时间：
2004-02-10
影响因子：
11.1
作者：
Ecker, DJ;Stein, P;Schultz, RM
通讯作者：
Schultz, RM
影响因子：
4.4
作者：
Kim, J;Bergmann, A;Stubbs, L
通讯作者：
Stubbs, L