Protein interactions within and between two F-type type IV secretion systems.
Protein interactions within and between two F-type type IV secretion systems.
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DOI：
10.1111/mmi.14582
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发表时间：
2020-11
影响因子：
3.6
通讯作者：
Krasnogor N
中科院分区：
文献类型：
--
作者：
Koch B;Callaghan MM;Tellechea-Luzardo J;Seeger AY;Dillard JP;Krasnogor N
细菌IV型分泌系统（T4 SS）可以介导接合。淋病奈瑟菌T4 SS具有独特的介导DNA分泌到细胞外环境的能力。了N.淋病T4 SS可根据同源性与F型接合T4 SS分组。我们测试了17种对N.淋病的蛋白质相互作用。BACTH-TM细菌双杂交系统已成功地用于研究周质相互作用。通过确定F-质粒T4 SS蛋白是否观察到相同的相互作用，以及当一个相互作用伴侣被另一个T4 SS的相应蛋白取代时，我们的目的是鉴定与N.淋病T4 SS以及F型T4 SS的一般特征。对于这两个系统，我们观察到已经描述的相互作用共享的同源物从其他T4 SS以及新的和描述的F型T4 SS特异性蛋白之间的相互作用。此外，我们证明，为第一次，与保守的T4 SS外膜核心蛋白和F型特异性蛋白质的同源性的蛋白质之间的相互作用，我们证实了其中两个通过共纯化。发现F型特异性蛋白TraHN定位于外膜，并且外膜中存在显著量的TraHN需要TraGN。
Bacterial type IV secretion systems (T4SSs) can mediate conjugation. The T4SS from Neisseria gonorrhoeae possesses the unique ability to mediate DNA secretion into the extracellular environment. The N. gonorrhoeae T4SS can be grouped with F-type conjugative T4SSs based on homology. We tested 17 proteins important for DNA secretion by N. gonorrhoeae for protein interactions. The BACTH-TM bacterial two-hybrid system was successfully used to study periplasmic interactions. By determining if the same interactions were observed for F-plasmid T4SS proteins and when one interaction partner was replaced by the corresponding protein from the other T4SS we aimed to identify features associated with the unique function of the N. gonorrhoeae T4SS as well as generic features of F-type T4SSs. For both systems, we observed already described interactions shared by homologs from other T4SSs as well as new and described interactions between F-type T4SS specific proteins. Furthermore, we demonstrate, for the first-time, interactions between proteins with homology to the conserved T4SS outer membrane core proteins and F-type specific proteins and we confirmed two of them by co-purification. The F-type specific protein TraHN was found to localize to the outer membrane and the presence of significant amounts of TraHN in the outer membrane requires TraGN.
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影响因子：
--
作者：
Christie PJ
通讯作者：
Christie PJ
影响因子：
3.6
作者：
Hamilton, HL;Domínguez, NM;Dillard, JP
通讯作者：
Dillard, JP
影响因子：
3.2
作者：
Hamilton, HL;Schwartz, KJ;Dillard, JP
通讯作者：
Dillard, JP
DOI：
10.1007/bf00299147
发表时间：
1992-03-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
FIRTH, N;SKURRAY, R
通讯作者：
SKURRAY, R
影响因子：
6.4
作者：
Gillespie JJ;Phan IQ;Scheib H;Subramanian S;Edwards TE;Lehman SS;Piitulainen H;Rahman MS;Rennoll-Bankert KE;Staker BL;Taira S;Stacy R;Myler PJ;Azad AF;Pulliainen AT
通讯作者：
Pulliainen AT