Genome-wide association study and transcriptome analysis dissect the genetic control of silique length in Brassica napus L.
Genome-wide association study and transcriptome analysis dissect the genetic control of silique length in Brassica napus L.
复制标题
全基因组关联研究和转录组分析剖析了甘蓝型油菜长角果长度的遗传控制。
DOI：
10.1186/s13068-021-02064-z
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发表时间：
2021-11-07
影响因子：
6.3
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Wang J;Fan Y;Mao L;Qu C;Lu K;Li J;Liu L
背景
油菜是仅次于大豆和棕榈的第三大油料作物，生产供人类消费的植物油和工业生产的生物燃料。角果长是油菜种子产量的重要性状，与产量密切相关。尽管在油菜中已经有许多与SL相关的研究报道，但目前只发现并克隆了少数候选基因，并且调控油菜SL的遗传机制仍不清楚。在这里，我们剖析了SL的遗传基础，全基因组关联研究（GWAS）结合转录组分析。
结果
我们确定了数量性状位点（QTL）的SL使用重组自交系（RIL）人口和两个独立的GWAS人口。A07、A09和C 08染色体上的主效QTL在所有群体的所有环境中都能稳定检测到。在主效QTL区间内检测到与淀粉和蔗糖代谢、植物激素信号传递和苯丙素合成相关的候选基因，如BnaA9.CP12-2、BnaA9.NST2、BnaA7.MYB63和BnaA7.ARF17。此外，RNA-seq和加权基因共表达网络分析（WGCNA）的结果表明，淀粉和蔗糖代谢，光合作用和次生细胞壁生物合成在角果发育中起着重要作用。
结论
光合作用、蔗糖和淀粉代谢、植物激素和木质素含量在油菜角果发育中起重要作用。
Background
Rapeseed is the third-largest oilseed crop after soybeans and palm that produces vegetable oil for human consumption and biofuel for industrial production. Silique length (SL) is an important trait that is strongly related to seed yield in rapeseed. Although many studies related to SL have been reported in rapeseed, only a few candidate genes have been found and cloned, and the genetic mechanisms regulating SL in rapeseed remain unclear. Here, we dissected the genetic basis of SL by genome-wide association studies (GWAS) combined with transcriptome analysis.
Results
We identified quantitative trait locus (QTL) for SL using a recombinant inbred line (RIL) population and two independent GWAS populations. Major QTLs on chromosomes A07, A09, and C08 were stably detected in all environments from all populations. Several candidate genes related to starch and sucrose metabolism, plant hormone signal transmission and phenylpropanoid biosynthesis were detected in the main QTL intervals, such as BnaA9.CP12-2, BnaA9.NST2, BnaA7.MYB63, and BnaA7.ARF17. In addition, the results of RNA-seq and weighted gene co-expression network analysis (WGCNA) showed that starch and sucrose metabolism, photosynthesis, and secondary cell wall biosynthesis play an important role in the development of siliques.
Conclusions
We propose that photosynthesis, sucrose and starch metabolism, plant hormones, and lignin content play important roles in the development of rapeseed siliques.
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影响因子：
4.5
作者：
Liu X;Huang M;Fan B;Buckler ES;Zhang Z
通讯作者：
Zhang Z
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
6.1
作者：
Chang, Xue Feng;Chandra, Richard;Beatson, Rodger P.
通讯作者：
Beatson, Rodger P.
影响因子：
4.6
作者：
Su J;Xu K;Li Z;Hu Y;Hu Z;Zheng X;Song S;Tang Z;Li L
通讯作者：
Li L
影响因子：
4.8
作者：
Fermani, Simona;Trivelli, Xavier;Trost, Paolo
通讯作者：
Trost, Paolo