Controlled ice nucleation using freeze-dried Pseudomonas syringae encapsulated in alginate beads.
Controlled ice nucleation using freeze-dried Pseudomonas syringae encapsulated in alginate beads.
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DOI：
10.1016/j.cryobiol.2017.03.006
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发表时间：
2017-04
期刊：
影响因子：
2.7
通讯作者：
Toner M
中科院分区：
文献类型：
--
作者：
Weng L;Tessier SN;Swei A;Stott SL;Toner M
冰核的控制在许多与食品和药物科学以及低温生物学相关的过程技术中具有重要意义。已知机械扰动、电磁场和冰成核剂（INA）以受控的方式诱导冰成核。但是这些冰成核方法可能遭受繁琐的手动操作、外部场的安全问题以及INA颗粒的生物相容性和回收问题，特别是当用于生命系统时。鉴于INA的自动冰播种性质，克服上述一些限制的一个有希望的解决方案是设计一种生物复合材料，该生物复合材料容纳INA颗粒，但最大限度地减少它们与生物制剂的相互作用，以及能够回收使用过的颗粒。在这项研究中，冷冻干燥的假单胞菌，模型冰成核剂，封装到微升大小的海藻酸盐珠。我们通过研究包括珠粒的尺寸和数量以及INA颗粒的局部浓度的因素来评估细菌水凝胶珠粒在水和甘油水溶液中引发冰成核的性能。在固定体积的水性样品中，发现INA颗粒的总质量（m）是唯一负责确定细菌水凝胶珠的冰成核性能的控制参数。冻结温度与log10m有很强的线性正相关关系。本研究的发现提供了一种有效的、可预测的控制冰核的方法，可以改善许多冰辅助工艺技术的结果和标准化。
The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice- nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleating methods may suffer from cumbersome manual operations, safety concerns of external fields, and biocompatibility and recovery issues of INA particles, especially when used in living systems. Given the automatic ice-seeding nature of INAs, a promising solution to overcome some of the above limitations is to engineer a biocomposite that accommodates the INA particles but minimizes their interactions with biologics, as well as enabling the recovery of used particles. In this study, freeze-dried Pseudomonas syringae, a model ice-nucleating agent, was encapsulated into microliter-sized alginate beads. We evaluated the performance of the bacterial hydrogel beads to initiate ice nucleation in water and aqueous glycerol solution by investigating factors including the size and number of the beads and the local concentration of INA particles. In the aqueous sample of a fixed volume, the total mass of the INA particles (m) was found to be the governing parameter that is solely responsible for determining the ice nucleation performance of the bacterial hydrogel beads. The freezing temperature has a strong positive linear correlation with log10m. The findings in this study provide an effective, predictable approach to control ice nucleation, which can improve the outcome and standardization of many ice- assisted process technologies.
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DOI：
10.1021/acs.jpclett.5b01096
发表时间：
2015-08-06
期刊：
The journal of physical chemistry letters
影响因子：
--
作者：
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通讯作者：
Salzmann CG
影响因子：
3.2
作者：
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通讯作者：
VONNEGUT, B
影响因子：
8.4
作者：
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Zhang, Zhihang
影响因子：
2.7
作者：
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通讯作者：
Glasmacher, Birgit
DOI：
10.1016/j.ijbiomac.2010.03.004
发表时间：
2010-06-01
影响因子：
8.2
作者：
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