Molecular characterization of oilseed rape accessions collected from multi continents for exploitation of potential heterotic group through SSR markers
Molecular characterization of oilseed rape accessions collected from multi continents for exploitation of potential heterotic group through SSR markers
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通过 SSR 标记对从多个大陆收集的油菜种质进行分子表征，以开发潜在的杂种优势群
DOI：
10.1007/s11033-011-1306-0
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发表时间：
2012-01
影响因子：
2.8
通讯作者：
Liu, Kede
中科院分区：
文献类型：
--
作者：
Younas, Muhammad;Xiao, Yingjie;Cai, Dongfang;Yang, Wei;Ye, Wei;Wu, Jiangsheng;Liu, Kede
评价传统和现代油菜籽品种的遗传多样性是保护、管理和利用这些遗传资源进行高产杂交种生产的重要依据。本研究利用188个SSR标记对86个油菜品种进行遗传变异、杂种优势群体认同和群体内、群体间关系的评价。在188个SSR标记上共检测到631个等位基因，其中独特等位基因53个，私有等位基因84个，表明所选品种遗传变异具有丰富度和独特性。每个微卫星位点的平均等位基因数为3.3个，平均多态性信息含量为0.35个。采用算术平均聚类和主成分分析相结合的非加权对群法将所有品种划分为4个不同的类群（I、II、III和IV），这与品种的地理分布基本一致。中国源品种主要集中在类群II中，具有较高的SSR位点等位基因丰度，遗传基础广泛；而大部分外来品种集中在类群I中，具有丰富的私有和独特等位基因，具有较高的差异性。遗传距离最高的是类群I和类群IV，分别以外来种黄籽1728-1和新合成黄籽品种G1087为主。本研究为进一步利用外源甘蓝型油菜在中国油菜育种中的应用提供了重要的见解。
Evaluation of the genetic diversity in conventional and modern rapeseed cultivars is essential for conservation, management and utilization of these genetic resources for high yielding hybrid production. The objective of this research was to evaluate a collection of 86 oilseed rape cultivars with 188 simple sequence repeat (SSR) markers to assess the genetic variability, heterotic group identity and relationships within and between the groups identified among the genotypes. A total of 631 alleles at 188 SSR markers were detected including 53 and 84 unique and private alleles respectively, which indicated great richness and uniqueness of genetic variation in these selected cultivars. The mean number of alleles per locus was 3.3 and the average polymorphic information content was 0.35 for all microsatellite loci. Unweighted Pair Group Method with Arithmetic Mean clustering and principal component analysis consistently divided all the cultivars into four distinct groups (I, II, III and IV) which largely coincided with their geographical distributions. The Chinese origin cultivars are predominantly assembled in Group II and showed wide genetic base because of its high allelic abundance at SSR loci while most of the exotic cultivars grouped into Group I and were highly distinct owing to the abundant private and unique alleles. The highest genetic distance was found between Group I and IV, which mainly comprised of exotic and newly synthesized yellow seeded (1728-1 and G1087) breeding lines, respectively. Our study provides important insights into further utilization of exoticBrassica napusaccessions in Chinese rapeseed breeding and vice versa.
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甘蓝型油菜中三个重复 PISTILLATA 基因的分离和表征
影响因子：
2.8
作者：
通讯作者：
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影响因子：
5.8
作者：
Seyis, F;Friedt, W;Lühs, W
通讯作者：
Lühs, W
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1.2
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DORRELL, DG;DOWNEY, RK
通讯作者：
DOWNEY, RK
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通讯作者：
Quiros, CF
影响因子：
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作者：
Riaz, A;Li, G;Quiros, CF
通讯作者：
Quiros, CF