Chondrocyte clusters adjacent to sites of cartilage degeneration have characteristics of progenitor cells.
Chondrocyte clusters adjacent to sites of cartilage degeneration have characteristics of progenitor cells.
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DOI：
10.1002/jor.22782
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发表时间：
2015-04
影响因子：
2.8
通讯作者：
Neo, Masashi
中科院分区：
文献类型：
--
作者：
Hoshiyama, Yoshiaki;Otsuki, Shuhei;Oda, Shuhei;Kurokawa, Yoshitaka;Nakajima, Mikio;Jotoku, Tsuyoshi;Tamura, Ryuichi;Okamoto, Yoshinori;Lotz, Martin K.;Neo, Masashi
本研究的目的是探讨软骨细胞簇在人膝关节骨性关节炎中的定位表达模式及其作用。取全膝关节置换术中股骨内侧和外侧内翻膝关节45个。以Stro-1、FGF2和Ki-67为抗体，通过活/死实验和免疫组织化学方法检测软骨退变、软骨细胞数和细胞排列情况。培养股骨内侧和外侧的软骨细胞，比较细胞的增殖能力和软骨结节的产生。最后，取股骨内侧髁部软骨组织，包括软骨裂隙和软骨细胞簇，观察其组织学变化。结果表明，重度退变附近的软骨细胞密度最高，而股骨外侧髁软骨细胞密度较低，细胞类型单一。这些软骨细胞群中80%以上存活，表达Stro-1、FGF2和Ki-67。此外，与单一类型的软骨细胞相比，软骨细胞群的增殖速度更快，产生的软骨结节更多。在器官培养过程中，含有大量软骨细胞簇的软骨裂隙被细胞外基质填充。综上所述，与重度软骨退变相邻的软骨细胞群表现出完全特异的特点，具有祖细胞和增殖潜能。调控软骨细胞簇可能为未来软骨修复和骨性关节炎的治疗提供新的途径。
The purpose of this study was to investigate the site-specific expression pattern and the role of chondrocyte clusters in human OA knee. Cartilage explants were obtained from 45 varus knees of medial and lateral femoral condyle undergoing total knee replacement surgery. Cartilage degeneration, number of chondrocytes, and the cell arrangement were evaluated by live/dead assay and immunohistochemical analyses with antibodies of STRO-1, FGF2, and Ki-67. Chondrocytes from medial and lateral femoral condyle were cultured to compare the potential of cell proliferation and production of cartilaginous nodules. Finally, cartilage tissue from medial femoral condyle, which included cartilage cleft with chondrocyte clusters, was observed the histological alternation. As the results, chondrocyte density adjacent to severe cartilage degeneration was highest, whereas chondrocytes in lateral femoral condyle displayed low density with single type of cells. Over 80% of these chondrocyte clusters were survived, expressing STRO-1, FGF2, and Ki-67. Furthermore, chondrocyte clusters proliferated faster and produced more cartilaginous nodules than single type of chondrocytes. Cartilage clefts involving numerous chondrocyte clusters were filled with extracellular matrix during organ culture. In conclusion, chondrocyte clusters adjacent to severe cartilage degeneration have shown completely specific characteristics with progenitor and proliferative potential. Regulating chondrocyte clusters may offer new approaches to cartilage repair and OA therapy in the future.
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影响因子：
--
作者：
Lotz, Martin K.;Otsuki, Shuhei;Grogan, Shawn P.;Sah, Robert;Terkeltaub, Robert;D'Lima, Darryl
通讯作者：
D'Lima, Darryl
影响因子：
7
作者：
Chang, C;Lauffenburger, DA;Morales, TI
通讯作者：
Morales, TI
影响因子：
--
作者：
Jones, EA;Kinsey, SE;McGonagle, D
通讯作者：
McGonagle, D
DOI：
10.1016/j.bbrc.2006.02.171
发表时间：
2006-05-05
影响因子：
3.1
作者：
Chiou, M;Xu, Y;Longaker, MT
通讯作者：
Longaker, MT
影响因子：
4.9
作者：
Grogan SP;Miyaki S;Asahara H;D'Lima DD;Lotz MK
通讯作者：
Lotz MK