The roles of DNA polymerases alpha, beta, and gamma in DNA repair synthesis induced in hamster and human cells by different DNA damaging agents.
The roles of DNA polymerases alpha, beta, and gamma in DNA repair synthesis induced in hamster and human cells by different DNA damaging agents.
复制标题
DNA 聚合酶 α、β 和 γ 在不同 DNA 损伤剂诱导的仓鼠和人类细胞 DNA 修复合成中的作用。
DOI：
10.1016/s0021-9258(18)34005-5
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
D. N. Chinault
中科院分区：
文献类型：
--
作者：
M. R. Miller;D. N. Chinault
在培养的仓鼠和人细胞的亚细胞制备物中研究了DNA聚合酶α、β和γ参与DNA修复合成。利用各种DNA损伤剂，包括博来霉素、新制癌素、UV照射和烷化剂来诱导DNA修复。修复合成的敏感性，以及复制合成和纯化的DNA聚合酶β活性，抑制DNA聚合酶抑制剂双脱氧胸苷三磷酸，阿非迪霉素，阿糖胞苷三磷酸，和N-乙基马来酰亚胺进行了测定。没有证据表明聚合酶γ在任何类型的修复合成中起主要作用。在仓鼠和人细胞中，博来霉素和新制癌素诱导的修复合成对ddTTP抑制的敏感性与纯化的聚合酶β观察到的基本相同，表明这些修复过程通过利用聚合酶β的机制进行。紫外线照射和烷化剂诱导的修复合成对ddTTP不敏感，表明这些病变的修复主要通过利用不同的DNA聚合酶（可能是聚合酶α）的途径发生。然而，复制合成是更敏感的聚合酶α抑制剂比紫外线照射或烷化剂诱导的修复合成。无论是DNA损伤的量还是诱导修复合成的量，都不会影响不同DNA聚合酶参与修复合成的程度。“补丁大小”或DNA损伤的实际类型的可能性，在何种程度上不同的聚合酶参与DNA修复合成进行了讨论。
The involvement of DNA polymerases alpha, beta, and gamma in DNA repair synthesis was investigated in subcellular preparations of cultured hamster and human cells. A variety of DNA damaging agents, including bleomycin, neocarzinostatin, UV irradiation, and alkylating agents, were utilized to induce DNA repair. The sensitivity of repair synthesis, as well as replicative synthesis and purified DNA polymerase beta activity, to inhibition by the DNA polymerase inhibitors dideoxythymidine triphosphate, aphidicolin, cytosine arabinoside triphosphate, and N-ethylmaleimide was determined. No evidence was obtained for a major role of polymerase gamma in any type of repair synthesis. In both hamster and human cells, the sensitivity of bleomycin- and neocarzinostatin-induced repair synthesis to ddTTP inhibition was essentially identical with that observed for purified polymerase beta, indicating these repair processes proceeded through a mechanism utilizing polymerase beta. Repair synthesis induced by UV irradiation and alkylating agents was not sensitive to ddTTP, indicating repair of these lesions occurred through a pathway primarily utilizing a different DNA polymerase; presumably polymerase alpha. However, replicative synthesis was much more sensitive to polymerase alpha inhibitors than was repair synthesis induced by UV irradiation or alkylating agents. Neither the amount of DNA damage nor the amount of induced repair synthesis influenced the degree to which the different DNA polymerases were involved in repair synthesis. The possibility that "patch size" or the actual type of DNA damage determines the extent to which different polymerases participate in DNA repair synthesis is discussed.
新制癌菌素、金霉素和博来霉素对脱氧核糖核酸的链断裂：碱基释放和核苷酸序列特异性的研究。
影响因子：
2.9
作者：
Takeshita,M;Kappen,LS;Grollman,AP;Eisenberg,M;Goldberg,IH
通讯作者：
Goldberg,IH
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Giloni,L;Takeshita,M;Johnson,F;Iden,C;Grollman,AP
通讯作者：
Grollman,AP
纯化的金霉素发色团导致脱氧核糖核酸链断裂时需要还原剂。
影响因子：
2.9
作者：
Kappen,LS;Napier,MA;Goldberg,IH;Samy,TS
通讯作者：
Samy,TS