Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level.
Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines owing to ped/pea-15 expression level.
复制标题
由于 ped/pea-15 表达水平，Omi/HtrA2 促凋亡标记物在各种肝细胞癌细胞系中存在差异。
DOI：
10.3892/or.2014.3656
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发表时间：
2015-02
期刊：
影响因子：
4.2
通讯作者：
Li Changhai
中科院分区：
文献类型：
--
作者：
Xu Zongquan;Chen Yu;Xu Guohui;Peng Cheng;Liu Enyu;Li Yunguang;Niu Jun;Li Changhai
OMI/HTRA2促进人类癌细胞中的细胞凋亡。早期研究表明，原发性肝细胞癌需要OMI/HTRA2表达细胞凋亡。此外，OMI/HTRA2促凋亡标记物在某些细胞T中显示出差异
Omi/HtrA2 promotes cell apoptosis in human cancer cells. Early studies showed that primary hepatocellular carcinoma requires Omi/HtrA2 expression for cell apoptosis. Additionally, the Omi/HtrA2 pro-apoptotic marker demonstrated a difference in some cell types. However, how the Omi/HtrA2 pro-apoptotic marker reacts during the process of hepatocellular carcinoma cell apoptosis remains to be determined. Thus, we investigated the role and possible mechanism of Omi/HtrA2 on hepatocellular carcinoma cell apoptosis using various hepatocellular carcinoma cell lines. The results were analyzed using RT‑qPCR and western blot analysis. In the present study, we found that Omi/HtrA2 was overexpressed in hepatocellular carcinoma cell lines and induced hepatocellular carcinoma cell apoptosis. Additiionally, the only manner in which Omi/HtrA2 participated in cell death in PLC cells may be dependent on IAP-binding. Omi/HtrA2‑inducing HepG2 cell apoptosis may mainly depend on its serine protease activity while both IAP-binding and its serine protease activity participated in Hep3B cell apoptosis. This result suggested that Omi/HtrA2 pro-apoptotic marker differs in various hepatocellular carcinoma cell lines. PLC cells were also devoid of the expression of ped/pea-15 as the substrate of Omi/HtrA2 serine protease while ped/pea-15 was overexpressed in HepG2 and Hep3B cells and ped/pea-15 expression was higher in HepG2 cells than that in Hep3B cells. These results showed that Omi/HtrA2 overexpression promotes hepatocellular carcinoma cell apoptosis and the ped/pea-15 expression level causes this difference of the Omi/HtrA2 pro-apoptotic marker in the various hepatocellular carcinoma cell lines.
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影响因子：
2.8
作者：
Hu, Xiao-Yong;Xu, Yue-Min;Zeng, Fu-Qing
通讯作者：
Zeng, Fu-Qing
影响因子：
64.8
作者：
Liu, ZH;Sun, CH;Fesik, SW
通讯作者：
Fesik, SW
影响因子：
--
作者：
Zongquan Xu;Xiao-ping Chen;Cheng Peng;E. Liu;Yunguang Li;Chang-hai Li;J. Niu
通讯作者：
Zongquan Xu;Xiao-ping Chen;Cheng Peng;E. Liu;Yunguang Li;Chang-hai Li;J. Niu
影响因子：
10.5
作者：
Yang, QH;Church-Hajduk, R;Du, CY
通讯作者：
Du, CY
影响因子：
11.2
作者：
H. Yamaguchi;K. Bhalla;Hong-Gang Wang
通讯作者：
H. Yamaguchi;K. Bhalla;Hong-Gang Wang