Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.
Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.
复制标题
热量和电晕电荷对体外 T 细胞系分子传递的综合影响。
DOI：
10.1371/journal.pone.0293035
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
随着治疗癌症的基因和免疫疗法的快速增加，需要有效地转染细胞。先前的研究表明，电转移可以提供一种非病毒的基因递送方法。电转移传统上依赖于对感兴趣的细胞施加直流脉冲。在这项研究中，电晕充电作为一种替代传统的方法，作为一种手段，创造必要的电场，通过电转移提供材料。目标是确定作为模型系统的人T细胞系的跨膜分子递送是否增加。在为该研究创建的新型培养皿中，还结合电晕充电应用检查了处理过程中高温（37、40、43和45°C）的影响。结果显示，在室温（~23°C）下用电晕充电处理细胞引起分子递送的统计学显著增加，同时保持活力。单独加热不会对分子递送产生统计学显著影响。与仅加热的对照相比，组合电晕充电处理和加热导致分子递送的统计学显著增加。当与在室温下处理的对照相比时，组合的电晕充电处理和加热至所有温度显示分子递送的统计学显著增加。
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery. Electrotransfer traditionally relies upon the application of direct current pulses to the cells of interest. Corona charge was investigated in this study as an alternative to traditional methods as a means of creating the electric field necessary to deliver materials via electrotransfer. The goal was to determine if there was an increase in molecular delivery across the membrane of a human T cell line used as a model system. In a novel dish created for the study, the effects of elevated temperatures (37, 40, 43, and 45°C) during the treatment process were also examined in combination with corona charge application. Results showed that treating cells with corona charge at room temperature (~23°C) caused a statistically significant increase in molecular delivery while maintaining viability. Heat alone did not cause a statistically significant effect on molecular delivery. Combined corona charge treatment and heating resulted in a statistically significant increase on molecular delivery compared to controls that were only heated. Combined corona charge treatment and heating to all temperatures when compared to controls treated at room temperature, showed a statistically significant increase in molecular delivery.
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影响因子：
3.8
作者：
Ogawa, Y;Morikawa, N;Nishimura, S
通讯作者：
Nishimura, S
影响因子：
5
作者：
Connolly, Richard J.;Hoff, Andrew M.;Jaroszeski, Mark J.
通讯作者：
Jaroszeski, Mark J.
影响因子：
4.1
作者：
Sakai, Y;Khajoee, V;Hara, T
通讯作者：
Hara, T
DOI：
10.1109/trpms.2019.2936667
发表时间：
2020-05-01
影响因子：
4.4
作者：
Stancampiano, A.;Chung, T-H;Robert, E.
通讯作者：
Robert, E.
DOI：
10.1038/mtm.2016.28
发表时间：
2016
期刊：
Molecular therapy. Methods & clinical development
影响因子：
--
作者：
Edelblute CM;Heller LC;Malik MA;Bulysheva A;Heller R
通讯作者：
Heller R