Robust, pleiotropic drug resistance 5 (Pdr5)-mediated multidrug resistance is vigorously maintained in Saccharomyces cerevisiae cells during glucose and nitrogen limitation.
Robust, pleiotropic drug resistance 5 (Pdr5)-mediated multidrug resistance is vigorously maintained in Saccharomyces cerevisiae cells during glucose and nitrogen limitation.
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DOI：
10.1093/femsyr/foy029
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发表时间：
2018-06-01
影响因子：
3.2
通讯作者：
Golin J
中科院分区：
文献类型：
--
作者：
Rahman H;Carneglia J;Lausten M;Robertello M;Choy J;Golin J
酿酒酵母具有复杂的营养感应程序，用于应对含有有限营养的恶劣环境。因此，酵母细胞可以生活在不同的环境中，包括动物，作为寄生虫或病原体。由于它们与其他分泌有毒化学物质的生物体生活在混合种群中，因此了解酵母细胞在营养胁迫期间是否保持功能性多药耐药机制是很有意义的。我们测量了Pdr5的活性，在限制营养物质的条件下，主要的酵母菌药物外排泵。我们表明，这种转运蛋白的稳态水平保持不变，即使在低浓度的葡萄糖和氮的生长过程中，二维凝胶电泳显示许多蛋白质的水平下降。我们还评估了罗丹明6G运输和三个异生素剂在丰富的（合成葡萄糖）和饥饿培养基的阻力。我们证明了Pdr5功能在这两组条件下都得到了有力的维持。即使在葡萄糖和氮供应低的情况下，多药耐药性也会在酵母细胞中维持。
Saccharomyces cerevisiae has sophisticated nutrient-sensing programs for responding to harsh environments containing limited nutrients. As a result, yeast cells can live in diverse environments, including animals, as a commensal or a pathogen. Because they live in mixed populations with other organisms that excrete toxic chemicals, it is of interest to know whether yeast cells maintain functional multidrug resistance mechanisms during nutrient stress. We measured the activity of Pdr5, the major Saccharomyces drug efflux pump under conditions of limiting nutrients. We demonstrate that the steady-state level of this transporter remains unchanged during growth in low concentrations of glucose and nitrogen even though two-dimensional gel electrophoresis revealed a decrease in the level of many proteins. We also evaluated rhodame 6G transport and resistance to three xenobiotic agents in rich (synthetic dextrose) and starvation medium. We demonstrate that Pdr5 function is vigorously maintained under both sets of conditions. Multidrug resistance is maintained in yeast cells even when glucose and nitrogen supplies are low.
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影响因子：
11.3
作者：
Conrad M;Schothorst J;Kankipati HN;Van Zeebroeck G;Rubio-Texeira M;Thevelein JM
通讯作者：
Thevelein JM
影响因子：
4.5
作者：
Johnson C;Kweon HK;Sheidy D;Shively CA;Mellacheruvu D;Nesvizhskii AI;Andrews PC;Kumar A
通讯作者：
Kumar A
影响因子：
3.5
作者：
Moye-Rowley, WS
通讯作者：
Moye-Rowley, WS
DOI：
10.1261/rna.044552.114
发表时间：
2014-06
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Vaidyanathan PP;Zinshteyn B;Thompson MK;Gilbert WV
通讯作者：
Gilbert WV
影响因子：
4.8
作者：
Golin, J;Ambudkar, SV;May, L
通讯作者：
May, L