Protein kinase A regulates gene-specific translational adaptation in differentiating yeast.
Protein kinase A regulates gene-specific translational adaptation in differentiating yeast.
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DOI：
10.1261/rna.044552.114
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Gilbert WV
中科院分区：
文献类型：
--
作者：
Vaidyanathan PP;Zinshteyn B;Thompson MK;Gilbert WV
虽然翻译调控被广泛认为对细胞分化程序有显着贡献，但很少有全基因组研究接近这个问题。在这里，作者使用核糖体分析和mRNA-seq来评估酵母中葡萄糖饥饿时全基因组的基因特异性翻译活性。有趣的是，在快速分裂和葡萄糖饥饿的细胞中，少数转录物构成了大部分翻译mRNA，尽管富集的mRNA的身份是不同的。此外，作者确定了由蛋白激酶A（PKA）特异性介导的翻译反应的特征。这些研究很好地揭示了“translatome”的增长状态专业化。细胞分化是由基因表达的协调调节变化驱动的。最近的发现表明，翻译和转录一样有助于调节蛋白质丰度，但翻译调节在细胞分化中的作用在很大程度上是未知的。在这里，我们研究翻译重编程酵母细胞适应葡萄糖的情况下，刺激，诱导侵入性丝状分化。使用核糖体足迹分析和RNA测序来测定基因特异性的全基因组翻译活性，我们表明，长期的葡萄糖戒断伴随着基因特异性的翻译效率的变化，显着影响大多数基因的表达。值得注意的是，在快速分裂和饥饿分化的细胞中，来自少数（<5%）基因的转录物构成了翻译mRNA的大部分，并且这些高度翻译的信息的身份在不同条件下几乎不重叠。此外，这两组消息受条件依赖的翻译特权的约束。因此，翻译的“管家”过程在细胞分化期间不保持恒定，而是高度适应不同的生长条件。通过比较葡萄糖饥饿的生长衰减应力，不诱导侵入性抑制，我们区分葡萄糖特异性的翻译反应介导的信号通过蛋白激酶A（PKA）。总之，这些发现揭示了高度的生长状态专业化的translatome和确定PKA作为一个重要的调节基因特异性翻译活动。
While translational regulation is widely thought to contribute significantly to cellular differentiation programs, there are few genome-wide studies approaching this question. Here, the authors use ribosome profiling and mRNA-seq to assess gene-specific translation activity genome-wide in response to glucose starvation in yeast. Interestingly, a minority of transcripts comprise the majority of translating mRNA in both rapidly dividing and glucose-starved cells, though the identities of the translationally enriched mRNAs are distinct. Additionally, the authors identify features of the translational response that are specifically mediated by protein kinase A (PKA). These studies nicely reveal growth-state specialization of the “translatome.” Cellular differentiation is driven by coordinately regulated changes in gene expression. Recent discoveries suggest that translation contributes as much as transcription to regulating protein abundance, but the role of translational regulation in cellular differentiation is largely unexplored. Here we investigate translational reprogramming in yeast during cellular adaptation to the absence of glucose, a stimulus that induces invasive filamentous differentiation. Using ribosome footprint profiling and RNA sequencing to assay gene-specific translation activity genome-wide, we show that prolonged glucose withdrawal is accompanied by gene-specific changes in translational efficiency that significantly affect expression of the majority of genes. Notably, transcripts from a small minority (<5%) of genes make up the majority of translating mRNA in both rapidly dividing and starved differentiating cells, and the identities of these highly translated messages are almost nonoverlapping between conditions. Furthermore, these two groups of messages are subject to condition-dependent translational privilege. Thus the “housekeeping” process of translation does not stay constant during cellular differentiation but is highly adapted to different growth conditions. By comparing glucose starvation to growth-attenuating stresses that do not induce invasive filamentation, we distinguish a glucose-specific translational response mediated through signaling by protein kinase A (PKA). Together, these findings reveal a high degree of growth-state specialization of the translatome and identify PKA as an important regulator of gene-specific translation activity.
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影响因子：
56.9
作者：
Ryan, Owen;Shapiro, Rebecca S.;Boone, Charles
通讯作者：
Boone, Charles
影响因子：
64.8
作者：
Ptacek, J;Devgan, G;Snyder, M
通讯作者：
Snyder, M
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
6.6
作者：
Tudisca, V.;Recouvreux, V.;Portela, P.
通讯作者：
Portela, P.
DOI：
10.1126/science.1215110
发表时间：
2012-02-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brar GA;Yassour M;Friedman N;Regev A;Ingolia NT;Weissman JS
通讯作者：
Weissman JS