Femtogram detection limits for biogenic amines using microbore HPLC with electrochemical detection

Femtogram detection limits for biogenic amines using microbore HPLC with electrochemical detection
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使用微孔 HPLC 和电化学检测对生物胺进行飞克检测限

DOI:
10.1016/0006-8993(84)90523-7
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发表时间:
1984
期刊:
影响因子:
2.9
通讯作者:
I. Mefford
I. Mefford
中科院分区:
医学3区
文献类型:
--
作者:
E. J. Caliguri;I. Mefford

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据报道,生物胺、血清素(5-HT)、5-羟基吲哚乙酸、5-羟基色氨酸、去甲肾上腺素、肾上腺素、多巴胺和3,4-二羟基苯乙酸(DOPAC)的检测限为50-200 fg。这是用微孔HPLC电化学检测完成的。在1-5 pg水平获得了1.6-8.1%的繁殖率。该方法的实用性被证明用于分析1 μg或更少的脑组织
Detection limits for biogenic amines, serotonin (5-HT), 5-hydroxyindoleacetic acid, 5-hydroxytryptophan, norepinephrine, epinephrine, dopamine and 3, 4-dihydroxyphenylacetic acid (DOPAC), of 50–200 fg are reported. This was accomplished using microbore HPLC with electrochemical detection. Reproducibility at the 1–5 pg level of 1.6–8.1% was obtained. The utility of this approach is demonstrated for analysis of brain tissues of 1 μg or less
DOI: 10.1016/0003-2697(80)90101-3
发表时间: 1980
影响因子: 2.9
作者:
Mefford,IN;Gilberg,M;Barchas,JD
通讯作者: Barchas,JD