Involvement of a TNF homologue in balancing the host immune system of Macrobrachium nipponense.
Involvement of a TNF homologue in balancing the host immune system of Macrobrachium nipponense.
复制标题
TNF 同源物参与平衡日本沼虾宿主免疫系统。
DOI：
10.1016/j.ijbiomac.2019.05.045
复制
发表时间：
2019-08
影响因子：
8.2
通讯作者：
Liu Fengsong
中科院分区：
文献类型：
--
作者：
Qin Nan;Tang Ting;Liu Xin;Xie Song;Liu Fengsong
在脊椎动物中，肿瘤坏死因子（tnf）是一种众所周知的细胞因子，参与多种生理和病理事件。在本研究中，我们从macrobrachiumnipponense中鉴定了一个新的tnf样基因（MnTNF），该基因编码一个与哺乳动物TNFSF成员TWEAK和EDA共享可检测序列的蛋白质（26-27%）。组织分布分析表明，MnTNF主要在神经组织中表达，在血细胞、鳃、肠和肌肉中表达水平相对较高，而在肝胰腺中几乎检测不到。在鳃中，MnTNF的mRNA和蛋白水平在维氏气单胞菌侵染后均显著上调。RNA干扰（RNAi）介导的MnTNF沉默导致抗菌肽（AMP） genecrustin的显著过表达，但另一AMP基因抗脂多糖因子（ALF）的不显著过表达，并在细菌攻击后显著抑制酚氧化酶（PO）的激活。同时，NF-κ b样因子（NF-κB-like factor generelish）的表达增加，而dorsaland stat的表达不受影响。我们推测MnTNF通过协调Imd通路参与调节AMP基因的表达和PO的能力。
In vertebrates, tumor necrosis factors (TNFs) are well-known cytokines involved in a diversity of physiological and pathological events. In the present work we identified a novel TNF-like gene (MnTNF) fromMacrobrachiumnipponense, which codes for a protein sharing detectable sequence identify (26–27%) to the mammalian TNFSF members, TWEAK and EDA. Tissue distribution analysis indicated that MnTNF was predominantly expressed in nervous tissue, and at relatively high level in haemocytes, gill, intestine and muscle, whereas was almost undetectable in hepatopancreas. In gill, MnTNF was significantly up-regulated at both mRNA as well protein levels uponAeromonas veroniichallenge. RNA interference (RNAi) mediated MnTNF silencing led to a significant overexpression of the antimicrobial peptide (AMP) genecrustin, but a non-significant overexpression of another AMP gene anti-lipopolysaccharide factor (ALF), and inhibited the activation of phenoloxidase (PO) significantly following bacterial challenge. Meanwhile, the expression of NF-κB-like factor generelishwas increased while that ofdorsalandSTATwas uninfluenced in MnTNF-depleted prawn. We suppose that MnTNF be involved in regulating the expression of AMP genes and the capacity of PO by coordinating with the Imd pathway.
登录
查看更多内容
影响因子：
7.3
作者：
Giubellino A;Shi ZD;Jenkins LM;Worthy KM;Bindu LK;Athauda G;Peruzzi B;Fisher RJ;Appella E;Burke TR;Bottaro DP
通讯作者：
Bottaro DP
DOI：
10.2307/25470688
发表时间：
2008-08
期刊：
The Biological Bulletin
影响因子：
--
作者：
Shaoguang Sun;Weiquan Liu;Jigui Wang;Shu-Yan Yang;Ling Gu;Yan Hong;Dan-Dan Shang-Dan;B. Wang;Xiaoming Su;S. Qi
通讯作者：
Shaoguang Sun;Weiquan Liu;Jigui Wang;Shu-Yan Yang;Ling Gu;Yan Hong;Dan-Dan Shang-Dan;B. Wang;Xiaoming Su;S. Qi
影响因子：
2.9
作者：
M. Pozzolini;S. Scarfì;S. Ghignone;F. Mussino;L. Vezzulli;C. Cerrano;M. Giovine
通讯作者：
M. Pozzolini;S. Scarfì;S. Ghignone;F. Mussino;L. Vezzulli;C. Cerrano;M. Giovine
影响因子：
64.5
作者：
Maecker, H;Varfolomeev, E;Ashkenazi, A
通讯作者：
Ashkenazi, A
影响因子：
2.9
作者：
T. Tang;Wenqian Li;Xiaofen Wang;Yinjian Wu;Fengsong Liu
通讯作者：
T. Tang;Wenqian Li;Xiaofen Wang;Yinjian Wu;Fengsong Liu