Adsorption of hepatitis C virus particles onto the dialyzer membrane.
Adsorption of hepatitis C virus particles onto the dialyzer membrane.
复制标题
丙型肝炎病毒颗粒吸附到透析膜上。
DOI：
10.1111/j.1525-1594.1997.tb00442.x
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发表时间：
2008
影响因子：
2.4
通讯作者：
Y. Irie
中科院分区：
文献类型：
--
作者：
H. Hayashi;K. Okuda;O. Yokosuka;S. Kobayashi;K. Yokozeki;Y. Ohtake;Y. Irie
最近发现，慢性血液透析患者每次透析后，血液中丙型肝炎病毒（HCV）RNA水平显著降低。本研究旨在阐明这种现象的机制。在两个具有高血清水平HCV RNA的患者中，通过透析器（TF-α，Teijin Co.，Tokyo，Japan）中使用聚合酶链反应分析病毒RNA。在透析结束时，用盐水洗涤过滤器，在盐水洗涤期间，取等分试样用于通过分支DNA方法定量RNA。HCV核心抗原定量作为病毒颗粒的量度，并且还测量血红蛋白以校正血液污染。清除血液后，用异硫氰酸胍洗涤滤器，并测定RNA的回收率。滤液不含可检测的RNA。透析后过滤器的盐水洗涤液含有大量RNA。用异硫氰酸胍洗涤经充分盐水洗涤的过滤器也回收了显著量的RNA。在盐水洗涤过程中，洗涤液中RNA的回收远远滞后于血红蛋白的回收，这表明前者吸附在滤膜上。洗涤液中RNA和HCV核心抗原的回收率不一致，前者的回收滞后于后者。这些结果表明，在透析过程中，病毒颗粒吸附在滤膜的内表面上。这些吸附的病毒颗粒中的一些可能被施加到血液中用于透析的液压破坏。
It was recently found that the blood level of hepatitis C virus (HCV) RNA is significantly reduced after each dialysis procedure in patients on chronic hemodialysis. This study was designed to elucidate the mechanism for this phenomenon. In two patients with high serum levels of HCV RNA, the filtrate through the dialyzer (TF-alpha, Teijin Co., Tokyo, Japan) was analyzed for viral RNA using the polymerase chain reaction. At the end of dialysis, the filter was washed with saline, and during the saline washing, aliquots were taken for quantification of RNA by the branched DNA method. The HCV core antigen was quantified as a measure of viral particles, and hemoglobin was also measured for correcting for blood contamination. After the clearance of the blood, the filter was washed with guanidinium isothiocyanate, and the recovery of RNA was measured. The filtrate did not contain detectable RNA. The saline washing of the filter after dialysis contained a significant amount of RNA. Washing with guanidinium isothiocyanate of the thoroughly saline washed filter also recovered a significant amount of RNA. During saline washing, the recovery of RNA in the washing was much delayed behind that of hemoglobin, suggesting the adsorption of the former onto the filter membrane. There was a discordant recovery of RNA and HCV core antigen in the washing, the recovery of the former being delayed behind that of the latter. These results indicate that viral particles are adsorbed onto the inner surface of the filter membrane during dialysis. Some of these adsorbed viral particles are perhaps destroyed by hydraulic pressure applied to blood for dialysis.
影响因子：
3.8
作者：
KAITO, M;WATANABE, S;KOHARA, M
通讯作者：
KOHARA, M