The Ah locus: Correlation of intranuclear appearance of inducer-receptor complex with induction of cytochrome P1-450 mRNA
The Ah locus: Correlation of intranuclear appearance of inducer-receptor complex with induction of cytochrome P1-450 mRNA
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Ah 基因座：诱导物-受体复合物的核内出现与细胞色素 P1-450 mRNA 诱导的相关性
DOI：
10.1016/0092-8674(82)90427-5
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
H. Eisen
中科院分区：
文献类型：
--
作者：
R. Tukey;R. Hannah;M. Negishi;D. Nebert;H. Eisen
Ah基因座调节外来化学物质如3-甲基胆蒽和2，3，7，8-四氯二苯并-p-二恶英对细胞色素PI-450的诱导。诱导过程由胞质Ah受体控制。研究了来自近交系C57 BL/6 N（Ah-/Ahb）小鼠、近交系DBA/SN（Ah-/Ahd）小鼠和来自（C57 BL/6 N x DBA/PN）F1 x DBA/SN回交的杂合子（Ahb/Ahd）和纯合子（Ahd/Ahd）的肝脏中的胞质和核Ah受体。在体内给予C3 H-1，6]-2，3，7，&四氯二苯并-对-二恶英（3 H-TCDD）后，Ahb/Anband Ahb/Ahd小鼠的细胞质和细胞核中可检测到受体; Ahd/Ahd小鼠的细胞质中无法检测到受体，但在细胞核中发现受体，其水平为Ahb/Ahb小鼠的四分之一到五分之一。用北方杂交和Rot分析法测定PI-450（23 s）mRNA的含量。三种可能的Ah基因型小鼠的细胞核中出现的~ 3 H-TCDD-Ah受体复合物的量与PI-450 mRNA的诱导量之间存在良好的剂量-反应关系（r= 0.99）。
The Ah locus regulates the induction of cytochrome PI-450 by foreign chemicals such as 3-methylcholanthrene and 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin. The induction process is controlled by the cytosolic Ah receptor. The cytosolic and nuclear Ah receptors were studied in the liver from inbred C57BL/6N (Ah”/Ahb) mice, inbred DBA/SN (Ah “/Ahd) mice and heterozygotes(Ahb/Ahd) and homozygotes (Ahd/Ahd) derived from the (C57BL/6N x DBA/PN) Fl x DBA/SN backcross. After C3H-1, 6]-2, 3, 7, &tetrachlorodibenzo-p-dioxin(3H-TCDD) is given in vivo, the receptor in Ahb/Anband Ahb/Ahd mice is detectable in the cytoplasm and nucleus; in Ahd/Ahdmice the receptor is not measurable in the cytosol, but is found in the nucleus at levels one fourth to one fifth of those in Ahb/Ahb mice. PI-450 (23s) mRNA content was estimated by Northern hybridization and by Rot analysis with a mouse P,-450 cloned cDNA. An excellent dose-response relationship (r= 0.99) was found between the amount of 3H-TCDD-Ah receptor complex appearing in the nucleus and the quantity of PI-450 mRNA induced in mice with all three possible Ah genotypes.
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核雌激素受体的解离动力学。
影响因子：
2.9
作者：
deBoer,W;Notides,AC
通讯作者：
Notides,AC
DOI：
10.1002/jsscb.380170202
发表时间：
1981
期刊：
Journal of supramolecular structure and cellular biochemistry
影响因子：
--
作者：
Weinstein,IB
通讯作者：
Weinstein,IB
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影响因子：
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作者：
Chan,L;Dugaiczyk,A;Means,AR
通讯作者：
Means,AR
羟基磷灰石固定的雌激素受体的解离动力学。
影响因子：
2.9
作者：
deBoer,W;Notides,AC
通讯作者：
Notides,AC