Mucosal immune response to RDEC-1 infection: study of lamina propria antibody-producing cells and biliary antibody
Mucosal immune response to RDEC-1 infection: study of lamina propria antibody-producing cells and biliary antibody
复制标题
对 RDEC-1 感染的粘膜免疫反应：固有层抗体产生细胞和胆汁抗体的研究
作者：
C. Mcqueen;E. Boedeker;M. Le;Y. Hamada;W. Brown
兔感染大肠埃希菌RDEC-1是一种有效的粘附性腹泻的动物模型。了解RDEC-1感染在兔体内诱导的保护性免疫，将为疫苗的设计提供有用的信息，以防止这种感染和其他粘膜附着生物的感染。因此，为了确定在RDEC-1原发感染过程中与细菌定植有关的特异性免疫球蛋白A分泌的时间进程和位置，我们用表达AF/R1粘附毛的RDEC-1感染兔，并将抗AF/R1抗体阳性细胞在肠粘膜中的位置与RDEC-1的腔定植和粘膜附着位置进行了比较。用酶联免疫吸附试验检测肠液和胆汁中的抗AF/R1抗体，用免疫组织化学方法检测RDEC-1与肠上皮细胞的结合部位。抗AF/R1菌毛抗体阳性细胞以近端肠(十二指肠和空肠)最多。相反，RDEC-1在远端肠(盲肠和结肠)的腔内定植和附着于上皮细胞的密度最高。在感染后1周，从所有水平的肠道收集的液体中，抗AF/R1抗体的含量大致相同。未稀释胆汁中的抗AF/R1抗体水平至少比肠道冲洗时高出两个数量级。尽管肠腔中存在大量微生物，但在腹泻病缓解之前，RDEC-1与上皮细胞的附着丧失。我们的研究表明，在RDEC-1感染期间，(I)粘膜抗AF/R1抗体分泌最多的部位位于RDEC-1腔内定植和附着最大部位的附近，(Ii)胆汁是肠腔内特异性抗体的主要来源，以及(Iii)干扰RDEC-1与上皮细胞的附着可能有助于疾病的缓解。
Infection of rabbits with Escherichia coli RDEC-1 is a useful model for diarrheal disease caused by mucosally attaching E. coli. Understanding of the protective immunity induced by RDEC-1 infection in rabbits should provide information useful in the design of vaccines for protection against this infection and other mucosally attaching organisms as well. Thus, to define the time course and location of specific immunoglobulin A secretion in relation to bacterial colonization during primary RDEC-1 infection, we infected rabbits with RDEC-1, which express AF/R1 adherence pili, and compared sites of anti-AF/R1 antibody-containing cells in the intestinal mucosa with the sites of luminal colonization and mucosal attachment of RDEC-1. Also, anti-AF/R1 antibodies in intestinal fluids and bile were measured by enzyme-linked immunosorbent assay, and attachment sites of RDEC-1 to the intestinal epithelium were determined by immunohistochemical examination. Anti-AF/R1 pilus antibody-containing cells were most numerous in the proximal intestine (duodenum and jejunum). In contrast, both luminal colonization and attachment of RDEC-1 to epithelial cells were densest in the distal intestine (cecum and colon). Anti-AF/R1 antibodies were present in approximately equal amounts in fluids collected from all levels of the gut after week 1 postinfection. Anti-AF/R1 antibody levels in undiluted bile exceeded those in gut flushes by at least 2 orders of magnitude. Loss of RDEC-1 attachment to epithelial cells preceded resolution of diarrheal illness despite the presence of large numbers of organisms in the intestinal lumen. Our studies indicate that during RDEC-1 infection (i) sites of greatest mucosal anti-AF/R1 antibody secretion are proximal to sites of maximal RDEC-1 luminal colonization and attachment, (ii) bile is a major source of specific antibodies in the intestinal lumen, and (iii) interference with RDEC-1 attachment to epithelial cells may permit resolution of disease.
DOI：
10.1073/pnas.87.20.7839
发表时间：
1990-10-01
影响因子：
11.1
作者：
JERSE, AE;YU, J;KAPER, JB
通讯作者：
KAPER, JB