KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species.
KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species.
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DOI：
10.3389/fphys.2021.752940
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发表时间：
2021
影响因子：
4
通讯作者：
Posnack NG
中科院分区：
文献类型：
--
作者：
Cooper BL;Gloschat C;Swift LM;Prudencio T;McCullough D;Jaimes R;Posnack NG
心脏光学成像，也称为光心动图，采用参数敏感的荧光染料对心脏组织成像，并解决心脏功能下的电和钙振荡。这项技术越来越多地与传统心电图结合使用，甚至作为传统心电图的替代品。在过去的几十年里，光学测绘已经成熟为心脏研究应用的“黄金标准”，然而光学信号的分析可能具有挑战性。尽管对软件工具和算法进行了改进，但分析大型光学数据集通常需要大量的编程专业知识，而数据分析可能既费力又耗时。为了应对这一挑战，我们开发了一个可访问的开源软件脚本，它不受任何基于订阅的编程语言的限制。所描述的软件是用python语言编写的，它被恰当地命名为“KairoSight”，参考了希腊语中的“时机”（Kairos），以及“看到”从心脏组织获得的电压和钙信号的能力。为了展示分析特征并突出物种差异，我们使用了收集的实验数据集，这些数据集来自哺乳动物心脏（langendorff灌注大鼠、豚鼠和猪），用RH237（跨膜电压）和Rhod-2， AM（细胞内钙）染色，以及用FluoVolt（膜电位）和Fluo-4， AM（钙指示剂）染色的人诱导多能干细胞衍生的心肌细胞（hiPSC-CM）。我们也证明了心脏对ryanodine （ryanodine受体调节剂）和异丙肾上腺素（β -肾上腺素能激动剂）的反应性，并强调了消融损伤后的区域差异。KairoSight可以被基础和临床科学家用于分析复杂的心脏光学测绘数据集，而不需要专门的计算机科学专业知识或专有软件。
Cardiac optical mapping, also known as optocardiography, employs parameter-sensitive fluorescence dye(s) to image cardiac tissue and resolve the electrical and calcium oscillations that underly cardiac function. This technique is increasingly being used in conjunction with, or even as a replacement for, traditional electrocardiography. Over the last several decades, optical mapping has matured into a “gold standard” for cardiac research applications, yet the analysis of optical signals can be challenging. Despite the refinement of software tools and algorithms, significant programming expertise is often required to analyze large optical data sets, and data analysis can be laborious and time-consuming. To address this challenge, we developed an accessible, open-source software script that is untethered from any subscription-based programming language. The described software, written in python, is aptly named “KairoSight” in reference to the Greek word for “opportune time” (Kairos) and the ability to “see” voltage and calcium signals acquired from cardiac tissue. To demonstrate analysis features and highlight species differences, we employed experimental datasets collected from mammalian hearts (Langendorff-perfused rat, guinea pig, and swine) dyed with RH237 (transmembrane voltage) and Rhod-2, AM (intracellular calcium), as well as human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) dyed with FluoVolt (membrane potential), and Fluo-4, AM (calcium indicator). We also demonstrate cardiac responsiveness to ryanodine (ryanodine receptor modulator) and isoproterenol (beta-adrenergic agonist) and highlight regional differences after an ablation injury. KairoSight can be employed by both basic and clinical scientists to analyze complex cardiac optical mapping datasets without requiring dedicated computer science expertise or proprietary software.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1152/ajpheart.00019.2011
发表时间：
2012-01-01
影响因子：
4.8
作者：
Costa, Andrea R.;Panda, Nikhil C.;Laurita, Kenneth R.
通讯作者：
Laurita, Kenneth R.