Homozygous and heterozygous GH transgenesis alters fatty acid composition and content in the liver of Amago salmon (Oncorhynchus masou ishikawae).
Homozygous and heterozygous GH transgenesis alters fatty acid composition and content in the liver of Amago salmon (Oncorhynchus masou ishikawae).
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DOI：
10.1242/bio.20121263
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发表时间：
2012-10-15
期刊：
影响因子：
2.4
通讯作者：
Mori T
中科院分区：
文献类型：
--
作者：
Sugiyama M;Takenaga F;Kitani Y;Yamamoto G;Okamoto H;Masaoka T;Araki K;Nagoya H;Mori T
本研究通过转生长激素（GH）基因Amago（Oncorhynchus masou ishikawae），将来自同一物种的血红蛋白GH 1基因与金属硫蛋白-B启动子融合，构建了转GH基因Amago（Oncorhynchus masou ishikawae），并比较了纯合（Tg/Tg）和杂合（Tg/+）的GH转基因Amago和野生型对照（+/+）的生理状况。先前，我们已经报道了与对照相比，GH转基因鱼的脂肪组织通常较小，并且Δ-6脂肪酰基去饱和酶基因在Tg/+鱼中下调。然而，脂肪酸（FA）的组成还没有被测量以前在这些鱼。本研究采用气相色谱法比较了肝脏中脂肪酸的组成和含量。共检测到11种FA。GH转基因Amago的饱和脂肪酸和单不饱和脂肪酸（SFA和MUFA）组成如肉豆蔻酸（14：0）、棕榈油酸（16：1 n-7）、顺式异油酸（cis-18：1 n-7）显著降低（P<0.05）。多不饱和脂肪酸（PUFAs）的组成，如亚油酸（18：2n-6）、花生四烯酸（20：4 n-6）和二十二碳五烯酸（22：5 n-3）显著增加（P<0.05）。与+/+鱼相比，GH转基因鱼的血糖和三酰甘油水平显著降低（P<0.05）。此外，对Tg/Tg和+/+鱼肝组织进行3′-tag数字基因表达谱分析，结果表明，激活乙酰辅酶A羧化酶（Acetyl-CoA carboxylase，ACC）的重要因子Mid 1相互作用蛋白1（Mid 1 ip 1）在Tg/Tg鱼中表达下调，而参与FA催化的基因表达上调，包括长链脂肪酸辅酶A连接酶1（ACSL 1）和酰基辅酶A氧化酶3（ACOX 3）。这些数据表明，肝组织从GH转基因Amago显示饥饿的葡萄糖和脂质代谢的改变，由于GH过表达。降低血糖可抑制Mid 1 ip 1的合成，导致FA的从头合成减少，导致SFA和MUFA的减少。这在GH转基因Amago中诱导ACSL 1和ACOX 3的表达以通过β-氧化产生能量。
Growth hormone (GH) transgenic Amago (Oncorhynchus masou ishikawae), containing the sockeye GH1 gene fused with metallothionein-B promoter from the same species, were generated and the physiological condition through lipid metabolism compared among homozygous (Tg/Tg) and heterozygous GH transgenic (Tg/+) Amago and the wild type control (+/+). Previously, we have reported that the adipose tissue was generally smaller in GH transgenic fish compared to the control, and that the Δ-6 fatty acyl desaturase gene was down-regulated in the Tg/+ fish. However, fatty acid (FA) compositions have not been measured previously in these fish. In this study we compared the FAs composition and content in the liver using gas chromatography. Eleven kinds of FA were detected. The composition of saturated and monounsaturated fatty acids (SFA and MUFA) such as myristic acid (14:0), palmitoleic acid (16:1n-7), and cis-vaccenic acid (cis-18:1n-7) was significantly (P<0.05) decreased in GH transgenic Amago. On the other hand, the composition of polyunsaturated fatty acids (PUFAs) such as linoleic acid (18:2n-6), arachidonic acid (20:4n-6), and docosapentaenoic acid (22:5n-3) was significantly (P<0.05) increased. Levels of serum glucose and triacylglycerol were significantly (P<0.05) decreased in the GH transgenics compared with +/+ fish. Furthermore, 3′-tag digital gene expression profiling was performed using liver tissues from Tg/Tg and +/+ fish, and showed that Mid1 interacting protein 1 (Mid1ip1), which is an important factor to activate Acetyl-CoA carboxylase (ACC), was down-regulated in Tg/Tg fish, while genes involved in FA catabolism were up-regulated, including long-chain-fatty-acid–CoA ligase 1 (ACSL1) and acyl-coenzyme A oxidase 3 (ACOX3). These data suggest that liver tissue from GH transgenic Amago showed starvation by alteration in glucose and lipid metabolism due to GH overexpression. The decrease of serum glucose suppressed Mid1ip1, and caused a decrease of de novo FA synthesis, resulting in a decrease of SFA and MUFA. This induced expression of ACSL1 and ACOX3 to produce energy through β-oxidation in the GH transgenic Amago.
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DOI：
10.1152/ajpendo.00004.2002
发表时间：
2003-10-01
影响因子：
5.1
作者：
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作者：
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影响因子：
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作者：
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影响因子：
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