The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus).
The mining and evolutionary investigation of AP2/ERF genes in pear (Pyrus).
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梨AP2/ERF基因的挖掘与进化研究
DOI：
10.1186/s12870-018-1265-x
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发表时间：
2018-03-20
影响因子：
5.3
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Li X;Tao S;Wei S;Ming M;Huang X;Zhang S;Wu J
在植物中，ERF基因参与多种调节途径，例如植物生长以及生物和/或非生物应激反应。虽然中国白色梨（“砀山酥梨”）基因组已经公布，但有关梨ERF家族的基因功能、进化历史和表达模式等方面的知识仍然有限。在我们的研究中，共鉴定了155个梨ERF家族成员。Ka和Ks值表明，全基因组重复（WGD）和分散重复有效地促进了梨ERF家族的扩展。基因结构和遗传学分析将PbrERF家族分为12个类群，并通过比较分析预测了它们的基因功能。利用野生和栽培梨果实在3个关键发育时期进行qRT-PCR以验证组III中7个基因的相对表达水平。野生样品中这些基因的表达量高于栽培样品，特别是在果实膨大期。脱水处理的梨幼苗的转录组数据进一步揭示了7个基因中的4个响应干旱条件。AP 2/ERF基因家族在梨中有很大的扩展。比较分析显示ERF基因在多个途径中发挥功能作用的可能性。在野生和栽培梨果实发育的不同阶段的表达分析表明，第III组基因可能参与非生物和/或生物胁迫。进一步的转录组数据进行干旱处理的幼苗验证了ERF基因在胁迫反应中的潜在作用。这些结果将为深入了解ERF家族在高等植物中的功能和进化提供有价值的参考。本文的在线版本（10.1186/s12870-018-1265-x）包含补充材料，可供授权用户使用。
In plants, ERF genes participate in a variety of regulatory pathways, such as plant growth and biotic and/or abiotic stress responses. Although the genome of Chinese white pear (‘Dangshansuli’) has been released, knowledge regarding the ERF family in pear, such as gene functions, evolutionary history and expression patterns, remains limited. In our study, a total of 155 members of ERF families were identified in pear (Pyrus bretschneideri). The Ka and Ks values suggested that whole-genome duplication (WGD) and dispersed duplication have effectively contributed to the expansion of the pear ERF family. Gene structure and phylogeny analysis divided the PbrERF family into 12 groups, and their gene functions were predicted by comparative analysis. qRT-PCR was carried out to verify the relative expression levels of 7 genes in group III using wild and cultivated pear fruits at three key developmental stages. Wild samples had higher expression of these genes than cultivated samples, especially at the enlarged fruit stage. The transcriptome data of pear seedlings subjected to dehydration treatment further revealed that 4 of the 7 genes responded to drought conditions. The AP2/ERF gene family is greatly expanded in pear. Comparative analysis revealed the probability of ERF genes performing functional roles in multiple pathways. Expression analysis at different stages of pear fruit development in wild and cultivated samples indicated that genes in group III might be involved in abiotic and/or biotic stresses. Further transcriptome data on seedlings subjected to drought treatment verified the potential role of ERF genes in stress response. These results will provide a valuable reference for understanding the function and evolution of the ERF family in higher plants. The online version of this article (10.1186/s12870-018-1265-x) contains supplementary material, which is available to authorized users.
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影响因子：
2.1
作者：
Du, Dongliang;Hao, Ruijie;Zhang, Qixiang
通讯作者：
Zhang, Qixiang
DOI：
10.1073/pnas.0900906106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Fawcett, Jeffrey A.;Maere, Steven;Van de Peer, Yves
通讯作者：
Van de Peer, Yves
DOI：
10.1073/pnas.94.11.5961
发表时间：
1997-05-27
影响因子：
11.1
作者：
Buttner, M;Singh, KB
通讯作者：
Singh, KB
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
中国白梨 (Pyrus bretschneideri) MYB 转录因子的全基因组鉴定、进化和功能分化
影响因子：
4.9
作者：
Li, Xiaolong;Xue, Cheng;Wu, Jun
通讯作者：
Wu, Jun