Physical Features of Intracellular Proteins that Moonlight on the Cell Surface.
Physical Features of Intracellular Proteins that Moonlight on the Cell Surface.
复制标题
细胞内蛋白质的物理特征，在细胞表面上月光。
DOI：
10.1371/journal.pone.0130575
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Jeffery CJ
中科院分区：
文献类型：
--
作者：
Amblee V;Jeffery CJ
兼职蛋白质包括多功能蛋白质的子集，其执行两种或更多种生物化学功能，所述生物化学功能不是由于基因融合、多个剪接变体、蛋白水解片段或混杂酶活性。本文描述的项目集中于兼职蛋白质的子集，其在细胞内具有典型的生化功能，并且在至少一个物种中在细胞表面上执行第二生化功能。该项目的目标是考虑这些兼职蛋白质的生物物理特征，以确定它们是否具有共同的特征或定义特征，这些特征可能表明为什么这些特定蛋白质在细胞表面上被采用为第二功能，或者这些蛋白质是否类似于典型的细胞内蛋白质。后者可能表明，在细胞表面发现的许多其他正常细胞内蛋白质也可能以这种方式兼职。我们已经确定了30种在细胞内和细胞表面具有不同功能的蛋白质。其中一些蛋白质被发现在多个物种的表面上月光，有时在不同物种中具有不同的细胞外功能，因此研究集中共有98种蛋白质。虽然多种细胞内蛋白质（酶、伴侣蛋白等）在大多数情况下，这些蛋白质被发现具有细胞内蛋白质的典型物理特性。在蛋白质组学实验中，在细菌病原体和其他生物体的表面也发现了许多其他细胞内蛋白质。很可能这些蛋白质中的许多在细胞表面也有兼职功能。已知的兼职蛋白质的数量和种类越来越多，这表明可能存在比以前认为的更多的兼职蛋白质，并且兼职可能是更多蛋白质的共同特征。
Moonlighting proteins comprise a subset of multifunctional proteins that perform two or more biochemical functions that are not due to gene fusions, multiple splice variants, proteolytic fragments, or promiscuous enzyme activities. The project described herein focuses on a sub-set of moonlighting proteins that have a canonical biochemical function inside the cell and perform a second biochemical function on the cell surface in at least one species. The goal of this project is to consider the biophysical features of these moonlighting proteins to determine whether they have shared characteristics or defining features that might suggest why these particular proteins were adopted for a second function on the cell surface, or if these proteins resemble typical intracellular proteins. The latter might suggest that many other normally intracellular proteins found on the cell surface might also be moonlighting in this fashion. We have identified 30 types of proteins that have different functions inside the cell and on the cell surface. Some of these proteins are found to moonlight on the surface of multiple species, sometimes with different extracellular functions in different species, so there are a total of 98 proteins in the study set. Although a variety of intracellular proteins (enzymes, chaperones, etc.) are observed to be re-used on the cell surface, for the most part, these proteins were found to have physical characteristics typical of intracellular proteins. Many other intracellular proteins have also been found on the surface of bacterial pathogens and other organisms in proteomics experiments. It is quite possible that many of those proteins also have a moonlighting function on the cell surface. The increasing number and variety of known moonlighting proteins suggest that there may be more moonlighting proteins than previously thought, and moonlighting might be a common feature of many more proteins.
登录
查看更多内容
影响因子：
2.8
作者：
Candela, Marco;Biagi, Elena;Brigidi, Patrizia
通讯作者：
Brigidi, Patrizia
影响因子：
5.8
作者：
Carneiro, CRW;Postol, E;Brentani, RR
通讯作者：
Brentani, RR
影响因子：
3.6
作者：
Bergmann, S;Wild, D;Hammerschmidt, S
通讯作者：
Hammerschmidt, S
影响因子：
5.8
作者：
Dosztányi, Z;Csizmok, V;Simon, I
通讯作者：
Simon, I
DOI：
10.1016/j.bbapap.2008.03.017
发表时间：
2008-07-01
影响因子：
3.2
作者：
Agarwal, Shivangi;Kulshreshtha, Parul;Bhatnagar, Rakesh
通讯作者：
Bhatnagar, Rakesh