Identification of amino acids within the second alpha helical domain of the human immunodeficiency virus type 1 Vpu that are critical for preventing CD4 cell surface expression.
Identification of amino acids within the second alpha helical domain of the human immunodeficiency virus type 1 Vpu that are critical for preventing CD4 cell surface expression.
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DOI：
10.1016/j.virol.2009.10.048
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发表时间：
2010-02-05
期刊：
影响因子：
3.7
通讯作者：
Stephens, Edward B.
中科院分区：
文献类型：
--
作者：
Hill, M. Sarah;Ruiz, Autumn;Schmitt, Kimberly;Stephens, Edward B.
关键词：
Human immunodeficiency virus type 1 (HIV-1) encodes for a Vpu protein, which interacts with CD4 resulting in its degradation. In this study, we examined the role of the ten amino acids within the predicted second α-helical domain of the subtype B Vpu cytoplasmic tail in CD4 down-modulation using a VpuEGFP reporter system. Our findings indicate that the invariant leucine at position 63 and, to a lesser extent, the valine at position 68 were required for CD4 down-modulation. Mutation of analogous L63 in Vpu proteins subtypes A2, B, C, D, and H also abolished CD4 down-modulation from the cell surface. Co-immunoprecipitation analysis revealed that L63A and V68A mutants were capable of binding CD4 and still retained the ability to interact with h-β-TrCP1. Taken together, these results indicate that amino acid substitutions in the second α-helical domain that retain the predicted structure and binding to h-β-TrCP1 can influence Vpu-mediated CD4 degradation.
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影响因子：
3.7
作者：
Hill, M. Sarah;Ruiz, Autumn;Stephens, Edward B.
通讯作者：
Stephens, Edward B.
影响因子：
64.8
作者：
COHEN, EA;TERWILLIGER, EF;HASELTINE, WA
通讯作者：
HASELTINE, WA
影响因子：
3.7
作者：
Paul, M;Jabbar, MA
通讯作者：
Jabbar, MA
影响因子：
5.4
作者：
SCHWARTZ, S;FELBER, BK;PAVLAKIS, GN
通讯作者：
PAVLAKIS, GN
影响因子：
3.7
作者：
Hout, DR;Gomez, ML;Stephens, EB
通讯作者：
Stephens, EB