(Pheo)melanin photosensitizes UVA-induced DNA damage in cultured human melanocytes.
(Pheo)melanin photosensitizes UVA-induced DNA damage in cultured human melanocytes.
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(Pheo) 黑色素对培养的人类黑素细胞中 UVA 诱导的 DNA 损伤具有光敏性。
DOI：
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发表时间：
1998
影响因子：
6.5
通讯作者：
R. Kolb
中科院分区：
文献类型：
--
作者：
E. Wenczl;Nico P.M. Smit;Stan Pavel;A. Schothorst;Govert P. Van der Schans;A. Timmerman;Len Roza;R. Kolb
黑色素在人类皮肤细胞中是否具有光保护和/或光敏作用的问题仍在争论中。为了评估黑色素在 UVA 照射下的作用，在人类黑素细胞中测量了 DNA 单链断裂 (ssb)，仅在两种不同浓度（碱性 (0.01 mM) 或高浓度 (0.2 mM)）L-酪氨酸（黑色素的主要前体）培养时产生的色素量有所不同。同时，测定细胞的嗜黑色素和总黑色素含量。对来自 I 型皮肤和 VI 型皮肤个体的两种黑素细胞培养物进行了相同的实验。首次研究了 UVA 诱导的基因毒性与 pheo-/总黑色素含量之间的相关性。我们观察到，在基础培养基中培养时，皮肤 VI 型黑素细胞含有比皮肤 I 型黑素细胞多 10 倍的总黑色素和约 7 倍的褐黑素。培养基中酪氨酸水平的升高导致两种黑素细胞培养物中嗜黑色素和总黑色素水平的增加；然而，皮肤 I 型黑素细胞的黑色素成分变得更加生成褐黑素，而皮肤 VI 型黑素细胞的黑色素成分保持不变。在基础培养基中培养的皮肤 VI 型黑素细胞表现出对 UVA 的非常高的敏感性（1.18 ssb / 10(10) Da / kJ / m2），这可能与其高的嗜黑素和总黑色素含量有关。然而，通过在高酪氨酸浓度下培养来增加黑色素含量后，它们的 UVA 敏感性并没有改变。相比之下，在基础培养基中培养时，皮肤 I 型黑色素细胞对 UVA 的敏感性较低（0.04 ssb / 10(10) Da / kJ / m2），但增加黑色素含量会导致其 UVA 敏感性增加 3 倍（0.13 ssb / 10(10) Da / kJ / m2）。这些结果表明，经 UVA 照射的培养人黑素细胞对其自身合成的发色团（最有可能是褐黑素和/或黑色素中间体）进行光敏化。
The question of whether melanins are photoprotecting and/or photosensitizing in human skin cells continues to be debated. To evaluate the role of melanin upon UVA irradiation, DNA single-strand breaks (ssb) were measured in human melanocytes differing only in the amount of pigment produced by culturing at two different concentrations, basic (0.01 mM) or high (0.2 mM), of L-tyrosine, the main precursor of melanin. In parallel, pheo- and total melanin contents of the cells were determined. Identical experiments were performed with two melanocyte cultures derived from a skin type I and a skin type VI individual. For the first time the correlation between UVA-induced genotoxicity and pheo-/total melanin content has been investigated. We observed that cultured in basic medium, the skin type VI melanocytes contained 10 times more total melanin and about seven times more pheomelanin than the skin type I melanocytes. Elevation of tyrosine level in the culture medium resulted in an increase of both pheo- and total melanin levels in both melanocyte cultures; however, the melanin composition of skin type I melanocytes became more pheomelanogenic, whereas that of skin type VI melanocytes remained the same. The skin type VI melanocytes cultured in basic medium demonstrated a very high sensitivity (1.18 ssb per 10(10) Da per kJ per m2) toward UVA that is probably related to their high pheo- and total melanin content. Their UVA sensitivity, however, did not change after increasing their melanin content by culturing at high tyrosine concentration. In contrast, the skin type I melanocytes demonstrated a low sensitivity (0.04 ssb per 10(10) Da per kJ per m2) toward UVA when cultured in basic medium, but increasing their melanin content resulted in a 3-fold increase in their UVA sensitivity (0.13 ssb per 10(10) Da per kJ per m2). These results demonstrate that UVA-irradiated cultured human melanocytes are photosensitized by their own synthesized chromophores, most likely pheomelanin and/or melanin intermediates.
DOI：
10.1016/0027-5107(91)90121-4
发表时间：
1991
期刊：
Mutation research
影响因子：
--
作者：
J. G. Peak;M. Peak
通讯作者：
J. G. Peak;M. Peak
影响因子：
2.9
作者：
WOOD, ML;DIZDAROGLU, M;ESSIGMANN, JM
通讯作者：
ESSIGMANN, JM
来自黑人和白人供体的培养人类黑素细胞对阳光和紫外线 A 辐射的敏感性不同。
DOI：
10.1111/1523-1747.ep12616151
发表时间：
1992
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Yohn,JJ;Lyons,MB;Norris,DA
通讯作者：
Norris,DA