Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems.
Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems.
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DOI：
10.1146/annurev.biophys.050708.133652
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发表时间：
2010
影响因子：
12.4
通讯作者：
Lim WA
中科院分区：
文献类型：
--
作者：
Bashor CJ;Horwitz AA;Peisajovich SG;Lim WA
活细胞是一个极其复杂的实体，预测性和定量地理解其功能是生物学接下来的重大挑战之一。我们对细胞的了解大多涉及它的组成部分，但在很大程度上，我们尚未破译这些部分是如何组织起来以产生复杂生理功能的。传统上，我们通过基因或化学手段干扰细胞网络来了解其组织方式。新兴的合成生物学学科为研究系统提供了一种额外的有力方法。通过重新排列构成现有网络的各个部分，我们能够深入了解网络的层级逻辑，并确定进化用以产生创新功能的模块化构建模块。此外，通过构建最简“玩具”网络，人们可以系统地探索网络空间（连接和参数）与功能空间（系统的生理行为）之间的关系。在此，我们概述了近期利用合成生物学方法研究细胞网络的组织和功能的工作，并描述了一个可用于探究不同系统设计原则的合成生物学工具包的愿景。
The living cell is an incredibly complex entity, and the goal of predictively and quantitatively understanding its function is one of the next great challenges in biology. Much of what we know about the cell concerns its constituent parts, but to a great extent, we have yet to decode how these parts are organized to yield complex physiological function. Classically, we have learned about the organization of cellular networks by perturbing them through genetic or chemical means. The emerging discipline of synthetic biology offers an additional, powerful way to study systems. By rearranging the parts that comprise existing networks, we can gain valuable insight into the hierarchical logic of the networks and identify the modular building blocks that evolution uses to generate innovative function. Additionally, by building minimal “toy” networks, one can systematically explore the relationship between network space (linkages and parameters) and functional space (the system's physiological behavior). Here, we outline recent work that uses synthetic biology approaches to investigate the organization and function of cellular networks, and describe a vision for a synthetic biology toolkit that could be used to interrogate the design principles of diverse systems.
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