Regulation of subtilisin-like protease prC expression by nematode cuticle in the nematophagous fungus Clonostachys rosea.
Regulation of subtilisin-like protease prC expression by nematode cuticle in the nematophagous fungus Clonostachys rosea.
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线虫角质层对枯草杆菌蛋白酶样蛋白酶 prC 表达的调节。
DOI：
10.1111/j.1462-2920.2010.02296.x
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Zhang KQ
中科院分区：
文献类型：
--
作者：
Huang XW;Zou CG;Yang JK;Tao N;Liu XY;Gan ZW;Tu HH;Liu WJ;Zhang KQ
食线虫真菌已被用作对抗寄生于植物和动物的线虫的生物防治剂。这些真菌在线虫感染过程中能分泌枯草杆菌蛋白酶样的胞外丝氨酸蛋白酶。这些枯草杆菌蛋白酶样丝氨酸蛋白酶的表达受氮源的调节，包括线虫表皮。然而，氮源诱导的这些丝氨酸蛋白酶的表达的机制还没有很好地理解。在这项研究中，我们调查了氮源的影响，表达的枯草杆菌蛋白酶样胞外蛋白酶，prC，在食草真菌Clonostachys rosea。prC的破坏减弱了真菌对线虫的感染，表明该基因作为毒力因子起作用。优选的氮源（谷氨酰胺、氨）对prC的基础表达的抑制发生在转录水平。相反，线虫表皮诱导prC在转录后水平的表达。谷氨酰胺、氨和丝氨酸蛋白酶抑制剂苯甲基磺酰氟（phenylmethylsulfonyl fluoride）可显著抑制线虫表皮prC的诱导表达。因此，活性PrC的存在（尽管在培养基中水平较低）可能对于线虫角质层进一步诱导该基因至关重要。此外，小分子量（<3kD）的线虫表皮降解产物能显著诱导prC基因的表达。氨抑制了C. rosea抗线虫，可能是通过抑制prC的表达。因此，在施用大量铵盐的农田中，食腐真菌可能不能很好地发挥生防作用。
Nematophagous fungi have been used as biological control agents against nematodes parasitic to plants and animals. These fungi can secret subtilisin-like extracellular serine proteases during the infection of nematodes. The expression of these subtilisin-like serine proteases is regulated by nitrogen sources, including nematode cuticle. However, the mechanisms underlying the nitrogen sources-induced expression of these serine proteases is not well understood. In this study, we investigated the effect of nitrogen sources on the expression of a subtilisin-like extracellular protease, prC, in the nematophagous fungus Clonostachys rosea. Disruption of prC attenuated infection of the fungus to nematodes, indicating that this gene functions as a virulence factor. The inhibition of basal expression of prC by the preferred nitrogen sources (glutamine, ammonia) occurred at the transcriptional level. In contrast, nematode cuticle induced the expression of prC at the post-transcriptional level. The inducible expression of prC by nematode cuticle was significantly suppressed by glutamine, ammonia and phenylmethylsulfonyl fluoride (an inhibitor of serine protease). Thus, the existence of active PrC, albeit at a low level in the medium, is probably essential for further induction of this gene by nematode cuticle. Moreover, the low molecule weight (< 3 kD) degradation products of nematode cuticle could significantly induce the expression of prC. Ammonia suppresses the virulence of C. rosea against nematodes, probably by inhibiting prC expression. Thus, the nematophagous fungi probably could not function well as biocontrol agents in fields fertilized with a large amount of ammonium salt.
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DOI：
10.1099/00221287-139-7-1643
发表时间：
1993-07
期刊：
Journal of general microbiology
影响因子：
--
作者：
Claude G. Lerner;Robert C. Goldman
通讯作者：
Claude G. Lerner;Robert C. Goldman
秀丽隐杆线虫的角质层：其隔离和部分特征。
影响因子：
7.8
作者：
Cox, G N;Kusch, M;Edgar, R S
通讯作者：
Edgar, R S
影响因子：
2.6
作者：
I. Orozco;L. Ortiz;M. Elorza;J. Ruiz-Herrera;R. Sentandreu
通讯作者：
I. Orozco;L. Ortiz;M. Elorza;J. Ruiz-Herrera;R. Sentandreu
影响因子：
2.8
作者：
Ahman, J;Ek, B;Tunlid, A
通讯作者：
Tunlid, A
影响因子：
3
作者：
Alexander R Schmidt;Heinrich Dörfelt;Vincent Perrichot
通讯作者：
Alexander R Schmidt;Heinrich Dörfelt;Vincent Perrichot