Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells.
Isolation and culture of adult ciliary epithelial cells, previously identified as retinal stem cells, and retinal progenitor cells.
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分离和培养成体睫状上皮细胞（先前被鉴定为视网膜干细胞）和视网膜祖细胞。
DOI：
10.1002/9780470151808.sc01h04s19
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发表时间：
2011
影响因子：
--
通讯作者：
Gualdoni S
中科院分区：
文献类型：
--
作者：
Gualdoni S
本单元中描述的方案提供了如何在培养中分离和扩增睫状体上皮细胞（CECs），以前鉴定为视网膜干细胞，来自成年小鼠眼睛，以及胚胎视网膜祖细胞（rpc）来自发育中的视网膜的详细信息。cec最初作为神经球在漂浮条件下培养，然后在单层培养中扩增。rpc在漂浮条件下培养。详细的方案视网膜分化，以及外源基因表达使用慢病毒也进行了描述。Protoc。干细胞生物学。19:1H.4.1‐1H.4.15。©2011 by John Wiley & Sons, Inc。
The protocols described in this unit provide detailed information on how to isolate and expand, in culture, ciliary epithelial cells (CECs), previously identified as retinal stem cells, from the adult mouse eye, and embryonic retinal progenitor cells (RPCs) from the developing retina. CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described.Curr. Protoc. Stem Cell Biol. 19:1H.4.1‐1H.4.15. © 2011 by John Wiley & Sons, Inc.
影响因子：
2.7
作者：
HOLLYFIELD, JG
通讯作者：
HOLLYFIELD, JG
新的提醒系统显着提高局部眼部治疗的依从性
DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
Lebriz Ersoy;M. Hermann;M. Diestelhorst
通讯作者：
M. Diestelhorst
影响因子：
46.9
作者：
Osakada, Fumitaka;Ikeda, Hanako;Takahashi, Masayo
通讯作者：
Takahashi, Masayo