Reconstitution of yeast microsomal lipid flip‐flop using endogenous aminophospholipids
Reconstitution of yeast microsomal lipid flip‐flop using endogenous aminophospholipids
复制标题
使用内源氨基磷脂重建酵母微粒体脂质触发器
DOI：
10.1016/s0014-5793(00)01684-7
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发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Peter Mayinger
中科院分区：
文献类型：
--
作者：
T. Nicolson;Peter Mayinger
The molecular basis of transbilayer movement or flipping of phospholipids in the endoplasmic reticulum is largely unknown. To circumvent the problems inherent to studies with artificial phospholipid analogs, we studied microsomal flip-flop of endogenous phosphatidylethanolamine in yeast. The transbilayer transport of phosphatidylethanolamine was measured in reconstituted proteoliposomes derived from microsomal detergent extracts. Our results demonstrate that flipping is protease sensitive but does not require metabolic energy. Our assay is the first to use the endogenous substrate of the so-called ‘flippase’ to study phospholipid translocation in endomembranes and may therefore be crucial for the understanding of the catalytic properties of this elusive enzyme.
影响因子：
16.6
作者：
G. Carman;S. A. Henry
通讯作者：
G. Carman;S. A. Henry