The auxiliary protein complex SaePQ activates the phosphatase activity of sensor kinase SaeS in the SaeRS two-component system of Staphylococcus aureus.
The auxiliary protein complex SaePQ activates the phosphatase activity of sensor kinase SaeS in the SaeRS two-component system of Staphylococcus aureus.
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DOI：
10.1111/j.1365-2958.2012.08198.x
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发表时间：
2012-10
影响因子：
3.6
通讯作者：
Bae T
中科院分区：
文献类型：
--
作者：
Jeong DW;Cho H;Jones MB;Shatzkes K;Sun F;Ji Q;Liu Q;Peterson SN;He C;Bae T
在细菌双组分调节系统（TCS）中，磷酸化反应调节剂的去磷酸化对于将激活的系统重置到激活前状态是必不可少的。然而，在金黄色葡萄球菌的主要毒力TCS SaeRS TCS中，反应调节剂SaeR的去磷酸化机制尚未确定。在这里，我们报告说，两个辅助蛋白的sae操纵子，SaeP和SaeQ，形成一个蛋白质复合物与传感器激酶SaeS和激活传感器激酶的磷酸酶活性。磷酸酶活性的有效活化需要SaeP和SaeQ两者的存在。当SaeP和SaeQ异位表达时，凝固酶（对磷酸化SaeR具有低亲和力的sae靶标）的表达大大降低，而α-溶血素（对磷酸化SaeR具有高亲和力的sae靶标）的表达没有降低，证明SaePQ对sae靶标基因表达的差异效应。当SaePQ的表达被废除时，大多数sae靶基因被诱导在升高的水平。由于SaeP和SaeQ的表达是由SaeRS TCS诱导的，这些结果表明SaeRS TCS通过负反馈机制返回到激活前状态。
In bacterial two-component regulatory systems (TCSs), dephosphorylation of phosphorylated response regulators is essential for resetting the activated systems to the pre-activation state. However, in the SaeRS TCS, a major virulence TCS of Staphylococcus aureus, the mechanism for dephosphorylation of the response regulator SaeR has not been identified. Here we report that two auxiliary proteins from the sae operon, SaeP and SaeQ, form a protein complex with the sensor kinase SaeS and activate the sensor kinase’s phosphatase activity. Efficient activation of the phosphatase activity required the presence of both SaeP and SaeQ. When SaeP and SaeQ were ectopically expressed, the expression of coagulase, a sae target with low affinity for phosphorylated SaeR, was greatly reduced, while the expression of alpha-hemolysin, a sae target with high affinity for phosphorylated SaeR, was not, demonstrating a differential effect of SaePQ on sae target gene expression. When expression of SaePQ was abolished, most sae target genes were induced at an elevated level. Since the expression of SaeP and SaeQ is induced by the SaeRS TCS, these results suggest that the SaeRS TCS returns to the pre-activation state by a negative feedback mechanism.
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影响因子：
2.8
作者：
Fritsch, Frederike;Mauder, Norman;Beier, Dagmar
通讯作者：
Beier, Dagmar
影响因子：
64.8
作者：
HUANG, ZJ;EDERY, I;ROSBASH, M
通讯作者：
ROSBASH, M
DOI：
10.1099/00221287-6-1-2-95
发表时间：
1952-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
DUTHIE, ES;LORENZ, LL
通讯作者：
LORENZ, LL
影响因子：
14.9
作者：
ASLANIDIS, C;DEJONG, PJ
通讯作者：
DEJONG, PJ
影响因子：
3.2
作者：
Grundling, Angelika;Schneewind, Olaf
通讯作者：
Schneewind, Olaf