The Arabidopsis transcriptional regulator DPB3-1 enhances heat stress tolerance without growth retardation in rice.
The Arabidopsis transcriptional regulator DPB3-1 enhances heat stress tolerance without growth retardation in rice.
复制标题
DOI：
10.1111/pbi.12535
复制
发表时间：
2016-08
影响因子：
13.8
通讯作者：
Yamaguchi-Shinozaki K
中科院分区：
文献类型：
--
作者：
Sato H;Todaka D;Kudo M;Mizoi J;Kidokoro S;Zhao Y;Shinozaki K;Yamaguchi-Shinozaki K
提高作物的耐热性是粮食安全面临的一个重要挑战，以促进适应全球变暖。在拟南芥中，转录调节因子DNA聚合酶II亚单位B3-1(DPB3-1)/核因子Y亚单位C10(NF-YC10)被报道为脱水反应元件结合蛋白2A(DREB2A)的正调控因子，并且DPB3-1的过表达增强了植物的耐热胁迫能力，而不会阻碍生长。在这里，我们证明了DPB3-1与水稻和大豆中的DREB2A同系物相互作用。对拟南芥和水稻叶肉原生质体的转录激活分析表明，DPB3-1及其水稻同源物OsDPB3-2是DREB2A同源物的正调控因子。在非胁迫条件下，过表达DPB3-1基因不影响水稻植株的生长和产量。此外，过表达DPB3-1的水稻表现出更强的耐热胁迫能力。基因芯片分析表明，在热胁迫条件下，DPB3-1过表达的水稻中许多热胁迫诱导基因表达上调。然而，在非胁迫条件下，使用构成启动子的DPB3-1的过表达对这些基因的表达几乎没有影响。这可能是因为DPB3-1是一个共激活因子，因此缺乏固有的转录活性。我们的结论是，DPB3-1是一种在非生物胁迫条件下特异发挥作用的辅助激活因子，可以用于提高作物的耐热胁迫能力，而不会对营养和生殖生长产生负面影响。
The enhancement of heat stress tolerance in crops is an important challenge for food security to facilitate adaptation to global warming. In Arabidopsis thaliana, the transcriptional regulator DNA polymerase II subunit B3‐1 (DPB3‐1)/nuclear factor Y subunit C10 (NF‐YC10) has been reported as a positive regulator of Dehydration‐responsive element binding protein 2A (DREB2A), and the overexpression of DPB3‐1 enhances heat stress tolerance without growth retardation. Here, we show that DPB3‐1 interacts with DREB2A homologues in rice and soya bean. Transactivation analyses with Arabidopsis and rice mesophyll protoplasts indicate that DPB3‐1 and its rice homologue OsDPB3‐2 function as positive regulators of DREB2A homologues. Overexpression of DPB3‐1 did not affect plant growth or yield in rice under nonstress conditions. Moreover, DPB3‐1‐overexpressing rice showed enhanced heat stress tolerance. Microarray analysis revealed that many heat stress‐inducible genes were up‐regulated in DPB3‐1‐overexpressing rice under heat stress conditions. However, the overexpression of DPB3‐1 using a constitutive promoter had almost no effect on the expression of these genes under nonstress conditions. This may be because DPB3‐1 is a coactivator and thus lacks inherent transcriptional activity. We conclude that DPB3‐1, a coactivator that functions specifically under abiotic stress conditions, could be utilized to increase heat stress tolerance in crops without negative effects on vegetative and reproductive growth.
登录
查看更多内容
影响因子：
7.7
作者：
Lozano-Durán R;Macho AP;Boutrot F;Segonzac C;Somssich IE;Zipfel C
通讯作者：
Zipfel C
DOI：
10.1093/dnares/dsr040
发表时间：
2012
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Maruyama K;Todaka D;Mizoi J;Yoshida T;Kidokoro S;Matsukura S;Takasaki H;Sakurai T;Yamamoto YY;Yoshiwara K;Kojima M;Sakakibara H;Shinozaki K;Yamaguchi-Shinozaki K
通讯作者：
Yamaguchi-Shinozaki K
影响因子：
4.9
作者：
Chen, Songbiao;Tao, Lizen;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang
影响因子：
4.4
作者：
Gómez-Porras JL;Riaño-Pachón DM;Dreyer I;Mayer JE;Mueller-Roeber B
通讯作者：
Mueller-Roeber B
影响因子：
6.7
作者：
Deryng, Delphine;Conway, Declan;Warren, Rachel
通讯作者：
Warren, Rachel