Identification of cis-acting promoter elements in cold- and dehydration-induced transcriptional pathways in Arabidopsis, rice, and soybean.
Identification of cis-acting promoter elements in cold- and dehydration-induced transcriptional pathways in Arabidopsis, rice, and soybean.
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DOI：
10.1093/dnares/dsr040
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yamaguchi-Shinozaki K
中科院分区：
文献类型：
--
作者：
Maruyama K;Todaka D;Mizoi J;Yoshida T;Kidokoro S;Matsukura S;Takasaki H;Sakurai T;Yamamoto YY;Yoshiwara K;Kojima M;Sakakibara H;Shinozaki K;Yamaguchi-Shinozaki K
对拟南芥、水稻和大豆三种植物的基因组进行了测序，并预测了它们的许多基因和启动子。在拟南芥中，参与低温和脱水反应基因表达的顺式作用启动子元件已被广泛分析；然而，水稻和大豆低温和脱水诱导基因中这种顺式作用启动子序列的特征仍有待阐明。在这项研究中，我们使用这三个物种进行了微阵列分析，并比较了已识别的冷诱导和脱水诱导基因的特征。这三个物种的冷反应基因和脱水反应基因的转录谱相似，表现为典型的上调(脱水素/LEA)和下调(光合作用相关)基因。研究了这三个物种启动子中的全部(46=4096)个六聚体序列，揭示了冷诱导和脱水诱导启动子中保守序列的频率。脱落酸反应元件(Abre)的一个核心序列在三个物种的脱水诱导启动子中最保守，这表明这三个物种对脱水诱导基因的转录调控是相似的，具有Abre依赖的转录途径。相反，对于冷诱导启动子，保守的六聚体序列在这三个物种中是不同的，这表明冷诱导基因在物种之间存在不同的转录调控途径。
The genomes of three plants, Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and soybean (Glycine max), have been sequenced, and their many genes and promoters have been predicted. In Arabidopsis, cis-acting promoter elements involved in cold- and dehydration-responsive gene expression have been extensively analysed; however, the characteristics of such cis-acting promoter sequences in cold- and dehydration-inducible genes of rice and soybean remain to be clarified. In this study, we performed microarray analyses using the three species, and compared characteristics of identified cold- and dehydration-inducible genes. Transcription profiles of the cold- and dehydration-responsive genes were similar among these three species, showing representative upregulated (dehydrin/LEA) and downregulated (photosynthesis-related) genes. All (46 = 4096) hexamer sequences in the promoters of the three species were investigated, revealing the frequency of conserved sequences in cold- and dehydration-inducible promoters. A core sequence of the abscisic acid-responsive element (ABRE) was the most conserved in dehydration-inducible promoters of all three species, suggesting that transcriptional regulation for dehydration-inducible genes is similar among these three species, with the ABRE-dependent transcriptional pathway. In contrast, for cold-inducible promoters, the conserved hexamer sequences were diversified among these three species, suggesting the existence of diverse transcriptional regulatory pathways for cold-inducible genes among the species.
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影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
DOI：
10.1073/pnas.96.26.15348
发表时间：
1999-12-21
影响因子：
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通讯作者：
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影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
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