Detection of factors that interact with the human β-interferon regulatory region in vivo by DNAase I footprinting
Detection of factors that interact with the human β-interferon regulatory region in vivo by DNAase I footprinting
复制标题
通过 DNAase I 足迹法检测体内与人 β-干扰素调节区相互作用的因子
DOI：
10.1016/0092-8674(86)90293-x
复制
发表时间：
1986
期刊：
影响因子：
64.5
通讯作者：
T. Maniatis
中科院分区：
文献类型：
--
作者：
K. Zinn;T. Maniatis
我们使用了DNAase I基因组足迹程序来检测细胞因子和人类β-干扰素基因调控序列之间的相互作用。在用Poly(I)-Poly(C)诱导之前，在−94和−167之间的一个区域和−68和−38之间的另一个区域检测到与DNA结合的因子。诱导后，这些因子解离，另一个因子结合到位于−77和−之间的区域。这些足迹与β-干扰素基因调节区缺失的影响的相关性(附文)表明，诱导前结合的因子是阻遏分子，而诱导后结合的成分是转录因子。诱导后抑制分子从dna上解离可能允许转录因子与β-干扰素启动子结合并激活。因此，β-干扰素基因可能受负调控机制的控制。
We have used a DNAase I genomic footprinting procedure to detect interactions between cellular factors and the regulatory sequences of the human β-interferon gene. Prior to induction with poly(I)-poly(C), factors that bind to DNA are detected in one region located between −94 and −167 from the mRNA cap site, and in another region located between −68 and −38. After induction these factors dissociate and another factor binds to a region located between −77 and −64. Correlation of these footprints with the effects of deletions in the regulatory region of the β-interferon gene (accompanying paper) suggest that the factors that bind prior to induction are repressor molecules, while the component that binds after induction is a transcription factor. Dissociation of the repressor molecules from the DNA after induction may allow the transcription factor to bind to and activate the β-interferon promoter. Thus, the β-interferon gene may be controlled by a negative regulatory mechanism.
影响因子：
56.9
作者：
EPHRUSSI, A;CHURCH, GM;GILBERT, W
通讯作者：
GILBERT, W
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
DOI：
10.1073/pnas.78.3.1416
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MORRISON, A;COZZARELLI, NR
通讯作者：
COZZARELLI, NR