Enhanced expression of WD repeat-containing protein 35 via nuclear factor-kappa B activation in bupivacaine-treated Neuro2a cells.
Enhanced expression of WD repeat-containing protein 35 via nuclear factor-kappa B activation in bupivacaine-treated Neuro2a cells.
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DOI：
10.1371/journal.pone.0086336
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Okada S
中科院分区：
文献类型：
--
作者：
Huang L;Kondo F;Harato M;Feng GG;Ishikawa N;Fujiwara Y;Okada S
WD重复序列蛋白家族包含大量蛋白质，并且参与多种细胞过程，如信号转导、细胞生长、增殖和凋亡。布比卡因是一种钠通道阻滞剂，用于局部浸润、神经阻滞、硬膜外和鞘内麻醉。最近，我们报道，布比卡因诱导活性氧（ROS）的产生和p38丝裂原活化蛋白激酶（MAPK）的激活，导致在小鼠神经母细胞瘤Neuro2a细胞中的WD重复蛋白35（WDR 35）的表达增加。研究表明，ROS通过磷酸化激活MAPK，随后激活核因子-κ B（NF-κ B）和激活蛋白1（AP-1）。本研究旨在检测NF-κ B和c-Jun/AP-1是否参与布比卡因诱导的Neuro2a细胞WDR 35表达。在Neuro2a细胞中，布比卡因激活NF-κ B和c-Jun。APDC是一种NF-κ B抑制剂，可减弱布比卡因处理的Neuro2a细胞中NF-κ B活性和WDR 35蛋白表达的增加。GW9662是一种选择性过氧化物酶体增殖物激活受体γ拮抗剂，可增强布比卡因处理的Neuro2a细胞中NF-κ B活性和WDR 35蛋白表达的增加。相反，c-Jun siRNA不抑制布比卡因诱导的WDR 35 mRNA表达的增加。这些结果表明，布比卡因诱导Neuro2a细胞中转录因子NF-κ B和c-Jun/AP-1的活化，而NF-κ B的活化参与布比卡因诱导的WDR 35表达的增加。
The family of WD repeat proteins comprises a large number of proteins and is involved in a wide variety of cellular processes such as signal transduction, cell growth, proliferation, and apoptosis. Bupivacaine is a sodium channel blocker administered for local infiltration, nerve block, epidural, and intrathecal anesthesia. Recently, we reported that bupivacaine induces reactive oxygen species (ROS) generation and p38 mitogen-activated protein kinase (MAPK) activation, resulting in an increase in the expression of WD repeat-containing protein 35 (WDR35) in mouse neuroblastoma Neuro2a cells. It has been shown that ROS activate MAPK through phosphorylation, followed by activation of nuclear factor-kappa B (NF-κB) and activator protein 1 (AP-1). The present study was undertaken to test whether NF-κB and c-Jun/AP-1 are involved in bupivacaine-induced WDR35 expression in Neuro2a cells. Bupivacaine activated both NF-κB and c-Jun in Neuro2a cells. APDC, an NF-κB inhibitor, attenuated the increase in NF-κB activity and WDR35 protein expression in bupivacaine-treated Neuro2a cells. GW9662, a selective peroxisome proliferator-activated receptor-γ antagonist, enhanced the increase in NF-κB activity and WDR35 protein expression in bupivacaine-treated Neuro2a cells. In contrast, c-Jun siRNA did not inhibit the bupivacaine-induced increase in WDR35 mRNA expression. These results indicate that bupivacaine induces the activation of transcription factors NF-κB and c-Jun/AP-1 in Neuro2a cells, while activation of NF-κB is involved in bupivacaine-induced increases in WDR35 expression.
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影响因子：
2.4
作者：
Harato M;Huang L;Kondo F;Tsunekawa K;Feng GG;Fan JH;Ishikawa N;Fujiwara Y;Okada S
通讯作者：
Okada S
影响因子：
5.8
作者：
Rahman, I;Marwick, J;Kirkham, P
通讯作者：
Kirkham, P
姜黄素通过改善 PPAR-γ 功能来保护神经元免受脑缺血引起的炎症。
DOI：
10.1155/2013/470975
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu ZJ;Liu W;Liu L;Xiao C;Wang Y;Jiao JS
通讯作者：
Jiao JS
影响因子：
3.7
作者：
Berisha SZ;Serre D;Schauer P;Kashyap SR;Smith JD
通讯作者：
Smith JD
影响因子：
5.1
作者：
Chen, Yu-Chang;Wu, Jui-Sheng;Lin, Teng-Nan
通讯作者：
Lin, Teng-Nan