Skin homeostasis during inflammation: a role for nerve growth factor.
Skin homeostasis during inflammation: a role for nerve growth factor.
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炎症期间的皮肤稳态：神经生长因子的作用。
DOI：
10.14670/hh-23.1
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发表时间：
2008
影响因子：
2
通讯作者：
L. Calzà
中科院分区：
文献类型：
--
作者：
S. Sivilia;M. Paradisi;G. D’Intino;Mercedes Fernández;S. Pirondi;L. Lorenzini;L. Calzà
皮肤是一个神经内分泌免疫器官，其中许多不同的分子以自分泌-旁分泌的方式运作，以保证组织在生理和病理生理条件下的稳态。在本文中，我们研究了NGF和p75受体的表达在皮肤中，在CFA诱导的炎症，在一个时间过程中的研究。采用免疫组化、图像定量分析、RT-PCR和Western blot等方法检测皮肤神经支配和增殖情况。自发性和诱发性疼痛行为也在实验大鼠进行了测量。主要研究结果如下：1）.如热痛觉过敏所示，在这种情况下发生外周感觉神经病，从而导致后爪皮肤的感觉去神经支配，如CGRP和PGP9.5-IR纤维的消失所示; 2）。在CFA炎症的急性期，皮肤（角质形成细胞）中的NGF和p75表达（mRNA和蛋白质）增加; 3）。在该阶段，如细胞周期相关蛋白Ki 67的表达所定义的，在皮肤中观察到更高的增殖活性; 4）。在长期慢性期，皮肤中NFG和p75表达进一步上调; 5）。trkA mRNA表达与p75和NGF mRNA表达呈负相关。这些结果表明，CFA慢性炎症从炎症演变为小纤维感觉神经病变，并且NGF似乎在这两个事件中起作用。
The skin is a neuroendocrine immune organ in which many different molecules operate in autocrine-paracrine manner to guarantee tissue homeostatsis in physiological and pathophysiological conditions. In this paper we examined NGF and p75 receptor expression in the skin, during CFA induced inflammation, in a time-course study. We also examined cutaneus innervation and proliferation, by means of immunohistochemistry and quantitative image analysis, RT-PCR and Western blot. Spontaneous and evoked pain-behavior was also measured in experimental rats. The main results can be summarized as follows: 1). a peripheral sensory neuropathy develops in this condition, as indicated by thermal hyperalgesia, thus leading to a sensory denervation of the hind-paw skin as indicated by disappearance of CGRP and PGP9.5-IR fibers; 2). NGF and p75 expression (mRNA and protein) increases in the skin (keratinocytes) in the acute phase of CFA inflammation; 3). at this stage, a higher proliferative activity is observed in the skin, as defined by the expression of cell cycle-associated protein Ki67; 4). in the long-lasting chronic phase there is a further up-regulation of NFG and p75 expression in the skin; 5). trkA mRNA expression inversely correlates with p75 and NGF mRNA expression. These results suggest that CFA chronic inflammation evolves from inflammation to a small fibers sensory neuropathy and NGF seems to play a role in both events.
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影响因子：
3.6
作者：
Lawman,MJ;Boyle,MD;Gee,AP;Young,M
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
10.1073/pnas.80.23.7215
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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DOI：
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发表时间：
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影响因子：
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作者：
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