Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.
Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.
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DOI：
10.3389/fcell.2021.796960
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发表时间：
2021
影响因子：
5.5
通讯作者：
Hubel A
中科院分区：
文献类型：
--
作者：
Li R;Walsh P;Truong V;Petersen A;Dutton JR;Hubel A
人诱导多能干细胞（hiPSC）的分化产生对细胞治疗和个性化医学有价值的细胞表型。这些hiPSC衍生的治疗产品的成功转化将依赖于在制造周期的多个阶段的有效冷冻保存。从低温生物学的角度来看，我们试图了解低温保存的挑战如何沿着沿着hiPSC到感觉神经元分化的轨迹在细胞表型之间演变。在三个不同阶段培养细胞以代表中间、分化和成熟的细胞产物。所有细胞阶段在不含二甲亚砜（DMSO）的制剂中保持≥90%的存活率，但冷冻前在DMSO中损失≥50%。拉曼光谱显示，在具有较低膜流动性的hiPSC衍生的神经元细胞中对过冷的敏感性较高，并且在具有较大细胞体的干细胞发育阶段中对次优冷却速率的敏感性较高。在无DMSO冷冻保存后获得高度存活和功能性的感觉神经元。我们的研究还表明，解离贴壁培养物在冻存后细胞的存活和功能方面起着重要作用。
Differentiation of human induced pluripotent stem cells (hiPSCs) generates cell phenotypes valuable for cell therapy and personalized medicine. Successful translation of these hiPSC-derived therapeutic products will rely upon effective cryopreservation at multiple stages of the manufacturing cycle. From the perspective of cryobiology, we attempted to understand how the challenge of cryopreservation evolves between cell phenotypes along an hiPSC-to-sensory neuron differentiation trajectory. Cells were cultivated at three different stages to represent intermediate, differentiated, and matured cell products. All cell stages remained ≥90% viable in a dimethyl sulfoxide (DMSO)-free formulation but suffered ≥50% loss in DMSO before freezing. Raman spectroscopy revealed higher sensitivity to undercooling in hiPSC-derived neuronal cells with lower membrane fluidity and higher sensitivity to suboptimal cooling rates in stem cell developmental stages with larger cell bodies. Highly viable and functional sensory neurons were obtained following DMSO-free cryopreservation. Our study also demonstrated that dissociating adherent cultures plays an important role in the ability of cells to survive and function after cryopreservation.
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DOI：
10.1016/j.mcn.2013.07.007
发表时间：
2013-09
期刊：
Molecular and cellular neurosciences
影响因子：
--
作者：
Burkhardt MF;Martinez FJ;Wright S;Ramos C;Volfson D;Mason M;Garnes J;Dang V;Lievers J;Shoukat-Mumtaz U;Martinez R;Gai H;Blake R;Vaisberg E;Grskovic M;Johnson C;Irion S;Bright J;Cooper B;Nguyen L;Griswold-Prenner I;Javaherian A
通讯作者：
Javaherian A
DOI：
10.3390/molecules22111789
发表时间：
2017-11-10
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
de Abreu Costa L;Henrique Fernandes Ottoni M;Dos Santos MG;Meireles AB;Gomes de Almeida V;de Fátima Pereira W;Alves de Avelar-Freitas B;Eustáquio Alvim Brito-Melo G
通讯作者：
Eustáquio Alvim Brito-Melo G
影响因子：
1.2
作者：
Khan, Sumbul Jawed;Abidi, Syeda Nayab Fatima;Smith-Bolton, Rachel K.
通讯作者：
Smith-Bolton, Rachel K.
影响因子：
8.8
作者：
Kondo, Takayuki;Imamura, Keiko;Inoue, Haruhisa
通讯作者：
Inoue, Haruhisa
影响因子：
2.7
作者：
Beier, A. F.;Schulz, J. D.;Zimmermann, H.
通讯作者：
Zimmermann, H.