Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes.
Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and TNF-α, IFN-γ, and IL-2 Cytokines Production in Cultures of Peripheral Blood Lymphocytes.
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DOI：
10.3390/molecules22111789
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发表时间：
2017-11-10
期刊：
影响因子：
--
通讯作者：
Eustáquio Alvim Brito-Melo G
中科院分区：
文献类型：
--
作者：
de Abreu Costa L;Henrique Fernandes Ottoni M;Dos Santos MG;Meireles AB;Gomes de Almeida V;de Fátima Pereira W;Alves de Avelar-Freitas B;Eustáquio Alvim Brito-Melo G
二甲基亚砜（DMSO）是一种两亲性分子，其极性结构域以亚砜基和两个非极性甲基为特征，因此它能够溶解极性和非极性物质并转置疏水屏障。DMSO被广泛用于治疗药物的增溶，研究表明，10% v/v的浓度对人外周血单核细胞（PBMC）的培养活性没有影响。然而，某些DMSO浓度可影响淋巴细胞活化并具有抗炎作用。因此，本研究的目的是评估DMSO对淋巴细胞活化参数的影响。用流式细胞术对6名健康受试者的PBMC进行细胞活力分析、增殖和细胞因子产生。结果表明，2.5% v/v DMSO浓度对淋巴细胞活力没有影响。1%和2% v/v浓度的DMSO降低了淋巴细胞的相对增殖指数，5%和10% v/v浓度的DMSO降低了总淋巴细胞、CD4+ T淋巴细胞和CD8+ T淋巴细胞的百分比，干扰素-γ (IFN-γ)、肿瘤坏死因子-α (TNF-α)和白细胞介素-2 （IL-2）的产生。由此可见，DMSO具有体外抗炎作用，其机制是通过降低淋巴细胞的活化，并表现为增殖减少和细胞因子产生减少。
Dimethylsulfoxide (DMSO) is an amphipathic molecule composed of a polar domain characterized by the sulfinyl and two nonpolar methyl groups, for this reason it is able to solubilize polar and nonpolar substances and transpose hydrophobic barriers. DMSO is widely used to solubilize drugs of therapeutic applications and studies indicated that 10% v/v concentration did not modify culture viability when used to treat human peripheral blood mononuclear cells (PBMC). However, some DMSO concentrations could influence lymphocyte activation and present anti-inflammatory effects. Therefore, the objective of this study was to evaluate the effect of DMSO on lymphocyte activation parameters. Cell viability analysis, proliferation, and cytokine production were performed on PBMC from six healthy subjects by flow cytometry. The results indicated that 2.5% v/v DMSO concentrations did not modify lymphocytes viability. DMSO at 1% and 2% v/v concentrations reduced the relative proliferation index of lymphocytes and at 5% and 10% v/v concentrations reduced the percentage of total lymphocytes, cluster of differentiation 4+ (CD4+) T lymphocytes and CD8+ T lymphocytes interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) producers. Thus, it was concluded that DMSO has an in vitro anti-inflammatory effect by reducing lymphocyte activation demonstrated with proliferation reduction and the decrease of cytokine production.
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DOI：
10.1590/s0100-879x2012007500139
发表时间：
2012-12
期刊：
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
影响因子：
--
作者：
Tossige-Gomes R;Avelar NC;Simão AP;Neves CD;Brito-Melo GE;Coimbra CC;Rocha-Vieira E;Lacerda AC
通讯作者：
Lacerda AC
影响因子：
3.7
作者：
Marthyn, P;Beuscart, A;Righi, M
通讯作者：
Righi, M
影响因子：
3.8
作者：
ANSEL, HC;LEAKE, WF
通讯作者：
LEAKE, WF
影响因子：
15
作者：
Notman, Rebecca;Noro, Massimo;Anwar, Jamshed
通讯作者：
Anwar, Jamshed
影响因子：
4
作者：
Huang SS;Chen CL;Huang FW;Hou WH;Huang JS
通讯作者：
Huang JS