In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity.
In Mycoplasma hominis the OppA-mediated cytoadhesion depends on its ATPase activity.
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DOI：
10.1186/1471-2180-11-185
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发表时间：
2011-08-19
期刊：
影响因子：
4.2
通讯作者：
Henrich B
中科院分区：
文献类型：
--
作者：
Hopfe M;Dahlmanns T;Henrich B
在人泌尿生殖道的兼性人类病原体人型支原体中，寡肽通透酶的底物结合结构域OppA是一种多功能蛋白质，参与营养摄取、细胞黏附和细胞外ATP的水解。
为了绘制与功能相关的蛋白质区域图谱，对OppA突变体的ATP酶活性和黏附行为进行了分析。沃克A和B基序的突变导致OppA的ATP酶活性抑制高达8%，而N端CS1或CS2区域（细菌OppA蛋白中保守的结构基序）的缺失使ATP酶活性降低至60%，与沃克B基序相邻的第三个保守区域CS3的缺失导致ATP酶活性降低至42%。
有趣的是，OppA突变体对固定的HeLa细胞的黏附表明，两个远端区域主要参与OppA的黏附：CS1区域，其缺失导致35%的细胞黏附；以及沃克A和B及其相邻的上游区域CS3，其缺失导致25%的细胞黏附。通过使用ATP酶抑制剂将支原体的细胞黏附降低至50%，证实了ATP酶活性对人型支原体对HeLa细胞黏附的影响。
这些发现表明，人型支原体OppA介导的细胞黏附既取决于相邻的CS1和ATP酶结构域区域的拓扑结构，也取决于胞外ATP酶活性的功能。
In Mycoplasma hominis, a facultative human pathogen of the human genital tract, OppA, the substrate-binding domain of the oligopeptide permease, is a multifunctional protein involved in nutrition uptake, cytoadhesion and hydrolysis of extracellular ATP. To map the function-related protein regions the ATPase activity and adhesive behavior of OppA mutants were analyzed. Mutations of the Walker BA motifs resulted in an inhibition of up to 8% of the OppA ATPase activity, whereas deletion of the N-terminal CS1 or the CS2 region, structural motifs that are conserved in bacterial OppA proteins, reduced ATPase activity to 60% and deletion of CS3, the third conserved region adjacent to the Walker B motif led to a reduction to 42% ATPase activity. Interestingly, adhesion of the OppA mutants to immobilized HeLa cells demonstrated that two distal regions are mainly involved in adherence of OppA: the CS1 region, deletion of which led to 35% of the cytoadhesion, and the Walker BA with the adjacent upstream region CS3, deletion of which led to 25% of the cytoadhesion. The influence of the ATPase activity on the adherence of M. hominis to HeLa cells was confirmed by the use of ATPase inhibitors which reduced mycoplasmal cytoadhesion to 50%. These findings suggest that the OppA-mediated cytoadherence of Mycoplasma hominis depends on both, the topology of the neighbouring CS1 and ATPase domain regions and the functionality of the ecto-ATPase activity in addition.
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影响因子：
3.2
作者：
Henrich, B;Hopfe, M;Hadding, U
通讯作者：
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10.1152/ajpcell.1997.273.3.c973
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通讯作者：
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