Administration of Oxygen Ultra-Fine Bubbles Improves Nerve Dysfunction in a Rat Sciatic Nerve Crush Injury Model.
Administration of Oxygen Ultra-Fine Bubbles Improves Nerve Dysfunction in a Rat Sciatic Nerve Crush Injury Model.
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DOI：
10.3390/ijms19051395
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发表时间：
2018-05-07
影响因子：
5.6
通讯作者：
Yoshikawa H
中科院分区：
文献类型：
--
作者：
Matsuoka H;Ebina K;Tanaka H;Hirao M;Iwahashi T;Noguchi T;Suzuki K;Nishimoto S;Murase T;Yoshikawa H
超细气泡（直径<200 nm）具有几种独特的特性，并已在各种医疗领域进行了测试。本研究的目的是探讨氧气超细气泡（OUB）对大鼠坐骨神经挤压伤（SNC）模型的影响。给大鼠腹腔注射1.5 mL生理盐水、稀释在生理盐水中的OUB或稀释在生理盐水中的氮气超细气泡（NUB），每周三次，共4周，分为四组：（1）对照组（假手术+生理盐水）;（2）SNC组（挤压+生理盐水）;（3）SNC+OUB组（挤压+OUB-生理盐水）;（4）SNC+NUB组（挤压+NUB-生理盐水）。采用连续稀释法观察OUB对体外培养的背根神经节（DRG）神经元和雪旺细胞（SC）的影响。与对照组相比，SNC组的坐骨神经功能指数、缩爪阈值、神经传导速度和有髓轴突显著降低; SNC+OUB组的这些参数显著改善，尽管NUB治疗不影响这些参数。在体外，OUB通过激活AKT信号显著促进DRG神经元的轴突生长，通过激活ERK 1/2和JNK/c-JUN信号显著促进SC增殖。OUB可能通过促进DRG神经元突起生长和SC增殖改善SNC大鼠的神经功能障碍。
Ultra-fine bubbles (<200 nm in diameter) have several unique properties and have been tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUBs) on a sciatic nerve crush injury (SNC) model rats. Rats were intraperitoneally injected with 1.5 mL saline, OUBs diluted in saline, or nitrogen ultra-fine bubbles (NUBs) diluted in saline three times per week for 4 weeks in four groups: (1) control, (sham operation + saline); (2) SNC, (crush + saline); (3) SNC+OUB, (crush + OUB-saline); (4) SNC+NUB, (crush + NUB-saline). The effects of the OUBs on dorsal root ganglion (DRG) neurons and Schwann cells (SCs) were examined by serial dilution of OUB medium in vitro. Sciatic functional index, paw withdrawal thresholds, nerve conduction velocity, and myelinated axons were significantly decreased in the SNC group compared to the control group; these parameters were significantly improved in the SNC+OUB group, although NUB treatment did not affect these parameters. In vitro, OUBs significantly promoted neurite outgrowth in DRG neurons by activating AKT signaling and SC proliferation by activating ERK1/2 and JNK/c-JUN signaling. OUBs may improve nerve dysfunction in SNC rats by promoting neurite outgrowth in DRG neurons and SC proliferation.
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影响因子：
3.4
作者：
Dayton, PA;Chomas, JE;Ferrara, KW
通讯作者：
Ferrara, KW
影响因子：
3.4
作者：
Apel, Peter J.;Ma, Jianjun;Callahan, Michael;Northam, Casey N.;Alton, Timothy B.;Sonntag, William E.;Li, Zhongyu
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Li, Zhongyu
DOI：
10.1083/jcb.201205025
发表时间：
2012-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fontana X;Hristova M;Da Costa C;Patodia S;Thei L;Makwana M;Spencer-Dene B;Latouche M;Mirsky R;Jessen KR;Klein R;Raivich G;Behrens A
通讯作者：
Behrens A
影响因子：
2.4
作者：
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通讯作者：
Park HT
影响因子：
29.4
作者：
Huang, Hsin-Yang;Liu, Hao-Li;Chen, You-Yin
通讯作者：
Chen, You-Yin