Primitive and definitive erythropoiesis in mammals.
Primitive and definitive erythropoiesis in mammals.
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哺乳动物中的原始和确定性红细胞生成。
DOI：
10.3389/fphys.2014.00003
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发表时间：
2014
影响因子：
4
通讯作者：
Palis J
中科院分区：
文献类型：
--
作者：
Palis J
红细胞（RBC）是人体内最丰富的细胞类型，有两种不同的类型-原始和确定。哺乳动物中的原始红细胞在胎儿和出生后作为较小的无核细胞循环，而原始红细胞在早期胚胎中作为大的有核细胞短暂循环，然后最终去核。两种细胞类型都是由谱系定型祖细胞形成的，所述谱系定型祖细胞产生一系列形态学上可识别的前体，所述前体去核以形成成熟RBC。虽然定形红细胞前体在胎儿肝脏和出生后骨髓中与巨噬细胞相关的血管外成熟，但原始红细胞前体在胚胎血流中作为半同步队列成熟。虽然细胞骨架网络对于维持细胞形状和定形红细胞的变形性至关重要，但对原始成红细胞中细胞骨架的组成和功能知之甚少。促红细胞生成素（EPO）是一个关键的调节剂，后期确定，但不是原始，红系祖细胞存活。然而，最近的研究表明，EPO调节原始小鼠和人类红系前体细胞的终末成熟的多个方面，包括细胞存活、增殖和终末成熟速率。原始和永久性红细胞生成共享中央转录调控因子，包括Gata 1和Klf 1，但也以其他调控因子的差异表达和功能为特征，包括myb，Sox 6和Bcl 11 A。基于流式细胞术的方法，开发纯化小鼠和人类阶段特异性红细胞前体，使比较全球基因表达研究，并提供新的见解红细胞成熟的生物学。
Red blood cells (RBCs), which constitute the most abundant cell type in the body, come in two distinct flavors- primitive and definitive. Definitive RBCs in mammals circulate as smaller, anucleate cells during fetal and postnatal life, while primitive RBCs circulate transiently in the early embryo as large, nucleated cells before ultimately enucleating. Both cell types are formed from lineage-committed progenitors that generate a series of morphologically identifiable precursors that enucleate to form mature RBCs. While definitive erythroid precursors mature extravascularly in the fetal liver and postnatal marrow in association with macrophage cells, primitive erythroid precursors mature as a semi-synchronous cohort in the embryonic bloodstream. While the cytoskeletal network is critical for the maintenance of cell shape and the deformability of definitive RBCs, little is known about the components and function of the cytoskeleton in primitive erythroblasts. Erythropoietin (EPO) is a critical regulator of late-stage definitive, but not primitive, erythroid progenitor survival. However, recent studies indicate that EPO regulates multiple aspects of terminal maturation of primitive murine and human erythroid precursors, including cell survival, proliferation, and the rate of terminal maturation. Primitive and definitive erythropoiesis share central transcriptional regulators, including Gata1 and Klf1, but are also characterized by the differential expression and function of other regulators, including myb, Sox6, and Bcl11A. Flow cytometry-based methodologies, developed to purify murine and human stage-specific erythroid precursors, have enabled comparative global gene expression studies and are providing new insights into the biology of erythroid maturation.
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影响因子：
2.6
作者：
Gallagher, Patrick G.
通讯作者：
Gallagher, Patrick G.
影响因子：
82.9
作者：
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影响因子：
20.3
作者：
Basu, Priyadarshi;Lung, Tina K.;Lloyd, Joyce A.
通讯作者：
Lloyd, Joyce A.
影响因子：
20.3
作者：
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通讯作者：
Palis, James
影响因子：
64.5
作者：
GEIDUSCHEK, JB;SINGER, SJ
通讯作者：
SINGER, SJ