Developmental Potential of Abnormally Fertilized Oocytes and the Associated Clinical Outcomes.
Developmental Potential of Abnormally Fertilized Oocytes and the Associated Clinical Outcomes.
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DOI：
10.3389/fphys.2020.528424
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发表时间：
2020
影响因子：
4
通讯作者：
Yu K
中科院分区：
文献类型：
--
作者：
Chen X;Shi S;Mao J;Zou L;Yu K
本研究旨在探讨延长无原核（0 PN）、多核（1 PN）和质量差的第3天胚胎的异常受精卵培养的胚胎发育潜力，并确定相关的临床结局。这是一项于2014年1月至2018年5月在金华市人民医院进行的回顾性研究。将正常发育的胚胎和异常的0 PN、1 PN和质量差的第3天胚胎培养至第5天或第6天用于胚胎移植。比较了异常胚胎和正常发育胚胎的临床结果。将来自831个处理周期的共计6466个胚胎（1542个0 PN、852个1 PN和4072个质量较差的第3天胚胎）培养至囊胚期。总囊胚形成率为17.3%（1121/6466），其中0 PN为18.2%，1 PN为26.1%，第3天胚胎质量差为15.2%。优质囊胚形成率为9.5%（616/6466），其中0 PN组为11.2%，1 PN组为14.8%，第3天胚胎质量差为7.8%。卵胞浆内单精子注射（ICSI）获得的0 PN和1 PN的囊胚率显著低于体外受精组。共有243个周期移植了来自异常胚胎的囊胚，导致109例（44.9%）临床妊娠和19例（17.4%）流产;对照组共有350个周期，导致214例（61.1%）临床妊娠和18例（8.4%）流产。异常胚胎组活产率明显低于对照组。总的来说，传统的体外受精产生的0 PN和1 PN受精卵，而不是ICSI，加上第3天的胚胎质量差，能够达到囊胚期，并产生公平的妊娠率和活产率。
This study aims to investigate the embryo development potential of extending the culture of abnormally fertilized zygotes with no pronuclear (0PN), monopronuclear (1PN), and poor-quality day 3 embryos and to determine the associated clinical outcomes. This is a retrospective study performed between January 2014 and May 2018 at Jinhua People’s Hospital. The normal developed embryos and the abnormal 0PN, 1PN, and poor-quality day 3 embryos were cultured to day 5 or 6 for embryo transfer. Clinical outcomes resulting from abnormal embryos and normally developed embryos were compared. A total of 6466 embryos (1542 0PN, 852 1PN, and 4072 poor-quality day 3 embryos) from 831 treatment cycles were cultured to the blastocyst stage. The total blastulation rate was 17.3% (1121/6466) with 18.2% in 0PN, 26.1% in 1PN, and 15.2% in poor-quality day 3 embryos. The rate for good-quality blastocyst formation was 9.5% (616/6466) with 11.2% in 0PN group, 14.8% in 1PN group, and 7.8% in poor-quality day 3 embryos, respectively. Blastulation rates of 0PN and 1PN derived from intracytoplasmic sperm injection (ICSI) were significantly lower compared with the in vitro fertilization group. A total of 243 cycles were transferred with blastocysts originating from abnormal embryos, resulting in 109 (44.9%) clinical pregnancies and 19 (17.4%) miscarriages; in the control group, a total of 350 cycles resulted in 214 (61.1%) clinical pregnancies and 18 (8.4%) miscarriages. The live birth rate was significantly lower in the abnormal embryo group than that in the control group. Collectively, conventional in vitro fertilization derived 0PN and 1PN zygotes, not ICSI, together with day 3 embryos with poor quality, that were able to reach the blastocyst stage and produce a fair pregnancy rate and live birth rate.
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DOI：
10.1016/j.ejogrb.2018.12.011
发表时间：
2019-02-01
影响因子：
2.6
作者：
Kovacs, Peter;Matyas, Szabolcs;Pribenszky, Csaba
通讯作者：
Pribenszky, Csaba
影响因子：
4
作者：
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通讯作者：
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
4
作者：
Balaban, Basak;Brison, Daniel;Van den Abbeel, Etienne
通讯作者：
Van den Abbeel, Etienne
影响因子：
4
作者：
Campbell, Alison;Fishel, Simon;Hickman, Cristina Fontes Lindemann
通讯作者：
Hickman, Cristina Fontes Lindemann