Influence of Dehydroxymethylepoxyquinomicin on Radiosensitivity of Thyroid Carcinoma TPC-1 Cells.
Influence of Dehydroxymethylepoxyquinomicin on Radiosensitivity of Thyroid Carcinoma TPC-1 Cells.
复制标题
去羟甲基环氧喹诺星对甲状腺癌TPC-1细胞放射敏感性的影响
作者：
探讨NF-κB抑制剂去羟甲基表氧喹诺霉素（DHMEQ）对甲状腺癌TPC-1细胞放射敏感性的影响。 使用免疫磁珠分选从TPC-1细胞中分离CD 133阳性细胞（CD 133 + TPC-1）和阴性细胞（CD 133-TPC-1）。采用MTT法和细胞克隆形成实验验证DHMEQ对细胞的毒性作用后，将细胞分为4组，分别给予DHMEQ、131 I照射和DHMEQ +131 I照射干预，第4组作为对照组。观察到细胞生长、凋亡和细胞周期的改变。 DHMEQ对TPC-1细胞有一定的毒性作用，IC_（50）为38.57 μg/mL（P < 0.05）。DHMEQ抑制照射后CD 133+和CD 133- TPC-1的增殖和克隆形成。DHMEQ +辐射对细胞生长的抑制率和细胞凋亡率均高于单独使用（P < 0.05），在相同的处理条件下，对CD 133-TPC-1的影响比CD 133 + TPC-1更明显（P < 0.05）。 DHEMQ可提高TC细胞对131 I的放射敏感性，抑制肿瘤细胞生长，促进肿瘤细胞凋亡。然而，与CD 133 − TPC-1相比，其对CD 133 + TPC-1的作用不太显著，这可能与CD 133+细胞的干细胞样特性有关。未来DHMEQ在TC 131 I放疗中的应用将有效提高患者的临床疗效。
To investigate the influence of dehydroxymethylepoxyquinomicin (DHMEQ), an NF-κB inhibitor, on radiosensitivity of thyroid carcinoma (TC) TPC-1 cells. The isolation of CDl33 positive cells (CD133+ TPC-1) and negative cells (CD133− TPC-1) from TPC-1 cells used immunomagnetic bead sorting. After verification of the toxicity of DHMEQ to cells by MTT and cell cloning assays, the cells were divided into four groups, of which three groups were intervened by DHMEQ, 131I radiation, and DHMEQ +131I radiation, respectively, while the fourth group was used as a control without treatment. Alterations in cell growth, apoptosis, and cell cycle were observed. DHMEQ had certain toxic effects on TPC-1 cells, with an IC50 of 38.57 μg/mL (P < 0.05). DHMEQ inhibited CD133+ and CD133- TPC-1 proliferation and their clonogenesis after irradiation. DHMEQ + radiation contributed to a growth inhibition rate and an apoptosis rate higher than either or them alone (P < 0.05), with a more significant effect on CD133− TPC-1 than CD133+ TPC-1 under the same treatment conditions (P < 0.05). DHEMQ can increase the radiosensitivity of TC cells to 131I, inhibit tumor cell growth, and promote apoptosis. However, its effect is less significant on CD133+ TPC-1 compared with CD133− TPC-1, which may be related to the stem cell-like properties of CD133+ cells. In the future, the application of DHMEQ in TC 131I radiotherapy will effectively improve the clinical effect of patients.
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影响因子：
2
作者：
Fujisaki, Hiroto;Nakano, Yutaka;Kitagawa, Yuko
通讯作者：
Kitagawa, Yuko
影响因子：
3.7
作者：
Mirshahidi, Saied;Simental, Alfred;Yuan, Xiangpeng
通讯作者：
Yuan, Xiangpeng
影响因子：
7.3
作者：
Barnabei L;Laplantine E;Mbongo W;Rieux-Laucat F;Weil R
通讯作者：
Weil R
DOI：
10.1016/j.biopha.2020.110569
发表时间：
2020-10-01
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
Rasmi, Rajan Radha;Sakthivel, Kunnathur Murugesan;Guruvayoorappan, Chandrasekaran
通讯作者：
Guruvayoorappan, Chandrasekaran
影响因子：
14.5
作者：
Fallahi, Poupak;Ferrari, Silvia Martina;Antonelli, Alessandro
通讯作者：
Antonelli, Alessandro