Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation.
Inhibitory effects of quail egg on mast cells degranulation by suppressing PAR2-mediated MAPK and NF-kB activation.
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鹌鹑蛋通过抑制 PAR2 介导的 MAPK 和 NF-kB 激活对肥大细胞脱颗粒的抑制作用
DOI：
10.29219/fnr.v62.1084
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发表时间：
2018
影响因子：
3.3
通讯作者：
Che H
中科院分区：
文献类型：
--
作者：
Lianto P;Ogutu FO;Zhang Y;He F;Che H
据报道，鹅蛋(QE)具有抗过敏和抗炎活性。我们已经证明，在食物过敏EoE小鼠模型中，全量QE能够减轻过敏症状，但QE蛋白和QE蛋黄中哪个起更重要的作用仍不清楚。在这项研究中，我们研究了QE在肥大细胞脱颗粒和效应时相反应中细胞因子产生的抑制作用。采用小鼠被动皮肤过敏反应(PCA)模型，验证QE的抗过敏作用。此外，利用HMC-1细胞模型对其抑制作用进行了更详细的研究。在这项体外研究中，我们将QE分为三组：完整QE组、QE蛋白组和QE蛋黄组。观察QE对肥大细胞脱颗粒和细胞内钙内流的影响。此外，还通过ELISA法、RT-PCR法和Western blotting法评估了QE过敏相关介质、基因和蛋白的作用。结果表明，全量QE(17 mg/kg)可显著降低IgE介导的PCA小鼠肥大细胞脱颗粒介导的耳部血管通透性，降低幅度达43.31±0.42%。基于HMC-1细胞的体外免疫实验表明，QE，尤其是其蛋白，起到了‘肥大细胞稳定剂’的作用。在70μg/mL浓度下，QE蛋白能有效抑制β-氨基己糖苷酶、组胺、类胰蛋白酶的释放，抑制Th2型细胞因子和促炎细胞因子的产生，降幅达30%~50%。此外，QE蛋白还能显著上调IL-10的表达，最高可达58.30±5.9%。有趣的是，我们的数据表明，QE蛋黄在其最高浓度(100μg/mL)时仍对Th2型细胞因子的调节有显著的抑制作用，而QE蛋白则没有抑制作用。Western印迹分析显示，QE蛋白有效地下调钙相关蛋白(TRPC1、ORAI1、STIM1、PLC-γ和IP3R)的表达，促进PAR-2的表达，并诱导JNK、IKKα、p50和p65蛋白的磷酸化水平降低。经PCA和HMC-1细胞免疫学检测证实，QE蛋白和QE蛋黄可能通过发挥抗过敏作用而发挥协同作用，是一种潜在的抗过敏营养物质。
Quail egg (QE) has been reported to possess an anti-allergic and anti-inflammatory activity. We have demonstrated that whole QE was able to attenuate the allergic symptoms in food allergy–induced EoE murine model, but whether QE albumen or QE yolk plays a more important role still remains unclear. In this current study, we investigated the suppressive role of QE in mast cell degranulation and cytokine production of the effect phase response. A passive cutaneous anaphylaxis (PCA) mouse model was used to confirm the anti-allergic effect of QE. Besides, HMC-1 cell model was used to study its suppressive role in more detail. In this in vitro study, we divided QE into three groups: whole QE, QE albumen, and QE yolk. The effect of QE treatment on mast cell degranulation and intracellular calcium influx was investigated. Moreover, the effect of QE allergy– related mediators, genes, and proteins were also assessed by ELISA, RT-PCR, and western blotting. Our data showed that the extent of mast cell degranulation–mediated ear vascular permeability in IgE-mediated PCA mice treated with whole QE (17 mg/kg) was decreased significantly up to 43.31 ± 0.42% reduction. HMC-1 cell–based immunological assay in vitro indicated that QE, particularly its albumen, acted as a ‘mast cell stabilizer’. Under the concentration of 70 μg/mL, QE albumen effectively suppressed the releases of β-hexosaminidase, histamine, and tryptase, as well as Th2 and pro-inflammatory cytokine production; reached 30 up to 50% reduction. Besides, QE albumen was also able to significantly modulate the upregulation of IL-10 up to 58.30 ± 5.9%. Interestingly, our data indicated that QE yolk still had a significant inhibitory effect on modulating Th2 cytokines in its highest concentration (100 μg/mL), while QE albumen showed no inhibitory effect. Western blot analysis showed QE albumen effectively down-regulated the expressions of calcium-related protein (TRPC1, Orai1, STIM1, PLC-γ and IP3R), facilitated the reduction of PAR-2 and induced the reduction of phosphorylation of JNK, IKKα, p50 and p65 protein expressions. As confirmed by PCA and HMC-1 cell-based immunology assay, QE albumen and QE yolk may work together through exerting anti-allergy activity and can be used as a potential anti-allergic nutrient in the future.
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影响因子：
3.9
作者：
Benichou, Annie-Claude;Armanet, Marion;Bussiere, Anthony;Chevreau, Nathalie;Cardot, Jean-Michel;Tetard, Jan
通讯作者：
Tetard, Jan
DOI：
10.1016/j.bbadis.2010.12.014
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Theoharides TC;Alysandratos KD;Angelidou A;Delivanis DA;Sismanopoulos N;Zhang B;Asadi S;Vasiadi M;Weng Z;Miniati A;Kalogeromitros D
通讯作者：
Kalogeromitros D
影响因子：
11.4
作者：
Huber, M;Helgason, CD;Krystal, G
通讯作者：
Krystal, G
影响因子：
3.9
作者：
Park, Gyu Hwan;Jeon, Se Jin;Ko, Kwang Ho
通讯作者：
Ko, Kwang Ho
影响因子：
8.7
作者：
Prakriya M
通讯作者：
Prakriya M