T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'.

T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'.
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T4相关的噬菌体石灰石分离株,用于控制由“ Dickeya Solani”引起的马铃薯的软腐烂。

DOI:
10.1371/journal.pone.0033227
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发表时间:
2012
期刊:
影响因子:
3.7
通讯作者:
Lavigne R
Lavigne R
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Adriaenssens EM;Van Vaerenbergh J;Vandenheuvel D;Dunon V;Ceyssens PJ;De Proft M;Kropinski AM;Noben JP;Maes M;Lavigne R

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"Dickeya solani“是Dickeya dianthicola的一个侵略性生物变种3,可引起马铃薯腐烂和黑胫病。为了使用噬菌体疗法控制这种病原体,我们分离并表征了两种密切相关的特异性噬菌体,vB_DsoM_LIMEstone 1和vB_DsoM_LIMEstone 2。石灰石噬菌体具有T4相关的基因组结构,并与沙门氏菌噬菌体VII共享DNA相似性。微生物学和分子生物学表征的噬菌体认为他们适合和有前途的噬菌体治疗中使用。在实验室测定中,该除草剂降低了马铃薯块茎上的病害发生率和严重程度。此外,在马铃薯块茎的田间试验中,当感染“Dickeya solani”时,实验性噬菌体处理导致更高的产量。这些结果形成了基于噬菌体的马铃薯植物和块茎的生物防治作为使用抗生素的替代品的发展的基础。
The bacterium ‘Dickeya solani’, an aggressive biovar 3 variant of Dickeya dianthicola, causes rotting and blackleg in potato. To control this pathogen using bacteriophage therapy, we isolated and characterized two closely related and specific bacteriophages, vB_DsoM_LIMEstone1 and vB_DsoM_LIMEstone2. The LIMEstone phages have a T4-related genome organization and share DNA similarity with Salmonella phage ViI. Microbiological and molecular characterization of the phages deemed them suitable and promising for use in phage therapy. The phages reduced disease incidence and severity on potato tubers in laboratory assays. In addition, in a field trial of potato tubers, when infected with ‘Dickeya solani’, the experimental phage treatment resulted in a higher yield. These results form the basis for the development of a bacteriophage-based biocontrol of potato plants and tubers as an alternative for the use of antibiotics.
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